Title:
METHOD FOR EXTRACTING SUBSTANCE FROM FECES SAMPLE
Document Type and Number:
WIPO Patent Application WO/2017/043087
Kind Code:
A1
Abstract:
The purpose of the present invention is to provide a more effective method for extracting substances from a feces sample. More specifically, the purpose of the present invention is to provide a method for extracting substances from a feces sample, the method being appropriate for performing analyses such as metabolomic analysis involving mass spectrography or nuclear magnetic resonance spectrometry, and metabolomic analysis involving a next-generation sequencer. A method for extracting one or more substances from a feces sample, the method characterized in comprising steps A-C below: step A: a step for acquiring a liquid suspension by suspending a feces sample in any of the following solvents (a)-(g): (a) a solvent containing an aqueous solvent and a hydrophilic organic solvent: (b) a solvent containing an aqueous solvent and a hydrophobic organic solvent: (c) a solvent containing an aqueous solvent, a hydrophilic organic solvent, and a hydrophobic organic solvent: (d) a solvent containing a hydrophilic organic solvent: (e) a solvent containing a hydrophobic organic solvent: (f) a solvent containing a hydrophilic organic solvent and a hydrophobic organic solvent: (g) a solvent containing water; step B: a step for collecting a liquid layer containing the solvent from the liquid suspension; and step C: a step for obtaining one or more substances after removing proteins from the liquid layer.
Inventors:
FUKUDA SHINJI (JP)
ISHII CHIHARU (JP)
TOMITA MASARU (JP)
ISHII CHIHARU (JP)
TOMITA MASARU (JP)
Application Number:
PCT/JP2016/004104
Publication Date:
March 16, 2017
Filing Date:
September 08, 2016
Export Citation:
Assignee:
UNIV KEIO (JP)
International Classes:
G01N1/28; C12Q1/04; C12Q1/24; G01N1/04; G01N1/10; C12Q1/68
Domestic Patent References:
| WO2010064634A1 | 2010-06-10 |
Foreign References:
| JP2012080853A | 2012-04-26 |
Other References:
MARCOBAL, A. ET AL.: "metabolomic view of how the human gut microbiota impacts the host metabolome using humanized and gnotobiotic mice", THE ISME JOURNAL, vol. 7, pages 1933 - 1943, XP055373532
MEGUMI SATO ET AL.: "Comparison of extraction methods of steroid hormones in feces", THE PROCEEDINGS OF NIHON UNIVERSITY FACULTY OF DENTISTRY, vol. 35, 2007, pages 49 - 56, XP009509014
YUMIKO NAKANISHI: "Evaluation of the effects of prebiotics on murine gut microbial ecology by multi-omic based network analysis", GRANTS-IN- AID FOR SCIENTIFIC RESEARCH KENKYU SEIKA HOKOKUSHO, 14 June 2015 (2015-06-14), XP055518771
HUMAN METABOLOME TECHNOLOGIES: "Metabolomics at HMT", THE PROCEEDINGS OF METABOLOMICS, 16 December 2011 (2011-12-16), XP009509013
MATSUMOTO, M. ET AL.: "Impact of Intestinal Microbiota on Intestinal Luminal Metabolome", NATURE SCIENTIFIC REPORTS, vol. 2, no. 233, 5 December 2012 (2012-12-05), pages 1 - 10, XP055518774
ROMICK-ROSENDALE, L.E. ET AL.: "NMR-based metabonomics analysis of mouse urine and fecal extracts following oral treatment with the broad-spectrum antibiotic enrofloxacin(Baytril", MAGNETIC RESONANCE IN CHEMISTRY, vol. 47, 18 September 2009 (2009-09-18), pages S36 - S46
HIROTAKA OBANA ET AL.: "Effect of Dietary Fiber on Fecal and Urinary Excretion of Trp-P-2 in the Rat", FOOD HYG. SAF. SCI., vol. 33, no. 5, October 1992 (1992-10-01), pages 437 - 441, XP055518780
AIKO UJIIE ET AL.: "Study on extraction of multiresidue pesticide analysis in fruit and vegetables", ANNUAL REPORT OF MIYAGI PREFECTURAL INSTITUTE OF PUBLIC HEALTH AND ENVIRONMENT, vol. 25, 2007, pages 58 - 61, XP009509017
See also references of EP 3348988A4
MEGUMI SATO ET AL.: "Comparison of extraction methods of steroid hormones in feces", THE PROCEEDINGS OF NIHON UNIVERSITY FACULTY OF DENTISTRY, vol. 35, 2007, pages 49 - 56, XP009509014
YUMIKO NAKANISHI: "Evaluation of the effects of prebiotics on murine gut microbial ecology by multi-omic based network analysis", GRANTS-IN- AID FOR SCIENTIFIC RESEARCH KENKYU SEIKA HOKOKUSHO, 14 June 2015 (2015-06-14), XP055518771
HUMAN METABOLOME TECHNOLOGIES: "Metabolomics at HMT", THE PROCEEDINGS OF METABOLOMICS, 16 December 2011 (2011-12-16), XP009509013
MATSUMOTO, M. ET AL.: "Impact of Intestinal Microbiota on Intestinal Luminal Metabolome", NATURE SCIENTIFIC REPORTS, vol. 2, no. 233, 5 December 2012 (2012-12-05), pages 1 - 10, XP055518774
ROMICK-ROSENDALE, L.E. ET AL.: "NMR-based metabonomics analysis of mouse urine and fecal extracts following oral treatment with the broad-spectrum antibiotic enrofloxacin(Baytril", MAGNETIC RESONANCE IN CHEMISTRY, vol. 47, 18 September 2009 (2009-09-18), pages S36 - S46
HIROTAKA OBANA ET AL.: "Effect of Dietary Fiber on Fecal and Urinary Excretion of Trp-P-2 in the Rat", FOOD HYG. SAF. SCI., vol. 33, no. 5, October 1992 (1992-10-01), pages 437 - 441, XP055518780
AIKO UJIIE ET AL.: "Study on extraction of multiresidue pesticide analysis in fruit and vegetables", ANNUAL REPORT OF MIYAGI PREFECTURAL INSTITUTE OF PUBLIC HEALTH AND ENVIRONMENT, vol. 25, 2007, pages 58 - 61, XP009509017
See also references of EP 3348988A4
Attorney, Agent or Firm:
HIROTA, Masanori (JP)
Download PDF:
Previous Patent: VERTICAL NARROW-GROOVE GAS SHIELDED ARC WELDING METHOD
Next Patent: INDUCTION HEATING POWER SUPPLY APPARATUS
Next Patent: INDUCTION HEATING POWER SUPPLY APPARATUS