Title:
METHOD FOR FERMENTING SUGARS USING GENETICALLY ENGINEERED YEAST
Document Type and Number:
WIPO Patent Application WO/2015/195934
Kind Code:
A3
Abstract:
A fermentation process for fermenting a starch hydrolysate containing 60-98 weight percent of glucose based on total carbohydrate and 0.3-5% weight percent of isomaltose based on total carbohydrate to a non-ethanol fermentation product is carried out. The method comprises: a) forming a fermentation broth containing the starch hydrolysate and a genetically modified microorganism containing i) an exogenous gene encoding transporter capable of transporting isomaltose ii) an exogenous gene encoding an enzyme capable of converting isomaltose to glucose; and b) fermenting the starch hydrolysate in the fermentation broth to produce a non-ethanol fermentation product. This fermentation process may be carried out as a single step fermentation or a batch process. The genetically modified microorganism may contain an exogenous gene encoding an enzyme that catalyzes the formation of a product other than ethanol.
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Inventors:
BRADY KEITH M (US)
MILLER CHRISTOPHER K (US)
MILLER CHRISTOPHER K (US)
Application Number:
PCT/US2015/036460
Publication Date:
February 25, 2016
Filing Date:
June 18, 2015
Export Citation:
Assignee:
CARGILL INC (US)
International Classes:
C12P19/02; C12P7/02; C12P7/40; C12P7/42; C12P7/56; C12P7/64; C12P13/04
Domestic Patent References:
| WO2008155665A2 | 2008-12-24 |
Foreign References:
| EP2241632A1 | 2010-10-20 |
Other References:
BOLES ECKHARD ET AL: "The molecular genetics of hexose transport in yeasts", FEMS MICROBIOLOGY REVIEWS, vol. 21, no. 1, 1997, pages 85 - 111, XP002745027, ISSN: 0168-6445
A. SMIT ET AL: "The Thr 505 and Ser 557 residues of the AGT1-encoded [alpha]-glucoside transporter are critical for maltotriose transport in Saccharomyces cerevisiae", JOURNAL OF APPLIED MICROBIOLOGY, vol. 104, no. 4, 1 April 2008 (2008-04-01), pages 1103 - 1111, XP055186801, ISSN: 1364-5072, DOI: 10.1111/j.1365-2672.2007.03671.x
TESTE M-A ET AL: "Characterization of a new multigene family encoding isomaltases in the yeast Saccharomyces cerevisiae, the IMA family", JOURNAL OF BIOLOGICAL CHEMISTRY 20100827 AMERICAN SOCIETY FOR BIOCHEMISTRY AND MOLECULAR BIOLOGY INC. USA, vol. 285, no. 35, 27 August 2010 (2010-08-27), pages 26815 - 26824, XP002745028, DOI: 10.1074/JBC.M110.145946
BADOTTI FERNANDA ET AL: "Switching the mode of sucrose utilization by Saccharomyces cerevisiae", MICROBIAL CELL FACTORIES, BIOMED CENTRAL, LONDON, NL, vol. 7, no. 1, 27 February 2008 (2008-02-27), pages 4, XP021036438, ISSN: 1475-2859
HAN E-K ET AL: "Characterization of AGT1 encoding a general alpha-glucoside transporter from Saccharomyces", MOLECULAR MICROBIOLOGY, WILEY-BLACKWELL PUBLISHING LTD, GB, vol. 17, no. 6, 1 January 1995 (1995-01-01), pages 1093 - 1107, XP003026803, ISSN: 0950-382X
A. SMIT ET AL: "The Thr 505 and Ser 557 residues of the AGT1-encoded [alpha]-glucoside transporter are critical for maltotriose transport in Saccharomyces cerevisiae", JOURNAL OF APPLIED MICROBIOLOGY, vol. 104, no. 4, 1 April 2008 (2008-04-01), pages 1103 - 1111, XP055186801, ISSN: 1364-5072, DOI: 10.1111/j.1365-2672.2007.03671.x
TESTE M-A ET AL: "Characterization of a new multigene family encoding isomaltases in the yeast Saccharomyces cerevisiae, the IMA family", JOURNAL OF BIOLOGICAL CHEMISTRY 20100827 AMERICAN SOCIETY FOR BIOCHEMISTRY AND MOLECULAR BIOLOGY INC. USA, vol. 285, no. 35, 27 August 2010 (2010-08-27), pages 26815 - 26824, XP002745028, DOI: 10.1074/JBC.M110.145946
BADOTTI FERNANDA ET AL: "Switching the mode of sucrose utilization by Saccharomyces cerevisiae", MICROBIAL CELL FACTORIES, BIOMED CENTRAL, LONDON, NL, vol. 7, no. 1, 27 February 2008 (2008-02-27), pages 4, XP021036438, ISSN: 1475-2859
HAN E-K ET AL: "Characterization of AGT1 encoding a general alpha-glucoside transporter from Saccharomyces", MOLECULAR MICROBIOLOGY, WILEY-BLACKWELL PUBLISHING LTD, GB, vol. 17, no. 6, 1 January 1995 (1995-01-01), pages 1093 - 1107, XP003026803, ISSN: 0950-382X
Attorney, Agent or Firm:
BJORKMAN, Dale A. et al. (PLLCSuite 200, Maple Island Building,221 Main Street Nort, Stillwater Minnesota, US)
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