Title:
METHOD FOR FLUORESCENTLY LABELING TARGET PROTEINS WITH HIGH EFFICIENCY USING PURIFIED FLUORESCENT METHIONINE AND EUKARYOTIC CELL TRANSLATION SYSTEM
Document Type and Number:
WIPO Patent Application WO/2017/105127
Kind Code:
A1
Abstract:
The present invention relates to a method for fluorescent labeling of target proteins with high efficiency by using purified fluorescent methionine and a eukaryotic cell translation system. More specifically, the present invention provides the effects of improving the synthesis efficiency of target proteins and the fluorescent labeling efficiency in vitro, by using purified fluorescent methionine for the synthesis of the target proteins in eukaryotic cells. In addition, the present invention can quantify a single fluorescently labeled amino acid through a quantitative curve of a single amino acid without derivatization of the single fluorescently labeled amino acid.
Inventors:
SEONG BAIK LIN (KR)
KIM JUNG MIN (KR)
KIM JUNG MIN (KR)
Application Number:
PCT/KR2016/014794
Publication Date:
June 22, 2017
Filing Date:
December 16, 2016
Export Citation:
Assignee:
UNIV-INDUSTRY FOUND YONSEI UNIV (KR)
International Classes:
C12N15/11; B01D15/32; B01D15/34; C12P21/00; G01N33/58; G01N33/68
Domestic Patent References:
| WO2011036666A2 | 2011-03-31 |
Foreign References:
| KR20090077243A | 2009-07-15 | |||
| KR20050099397A | 2005-10-13 | |||
| JP5049136B2 | 2012-10-17 |
Other References:
MURANAKA: "Incorporation of unnatural non-alpha-amino acids into the N terminus of proteins in a cell-free translation system", CHEMBIOCHEM, vol. 8, 2007, pages 1650 - 1653
Attorney, Agent or Firm:
DANA PATENT LAW FIRM (KR)
Download PDF: