Title:
METHOD OF FORMING AUTOAGGREGATE ON MICROPARTICLE AND METHOD OF DETECTING TARGET ANALYTE
Document Type and Number:
WIPO Patent Application WO/2007/037282
Kind Code:
A1
Abstract:
It is intended to provide a method whereby the sensitivity in detecting a target
analyte on a microparticle can be elevated and multiple items can be detected
at the same time. The presence of at least one target analyte in a sample is detected
by bonding a microparticle having a fluorescent substance on the surface and
the target analyte to an autoaggregate formed by using two oligonucleotide probes
having complementary base sequence regions which are hybridizable with each
other via the target analyte, thus forming an autoaggregate-bonded particle,
and then analyzing the autoaggregate-bonded particle.
Inventors:
FUJIKAWA TOSHIHIKO (JP)
USUI MITSUGU (JP)
USUI MITSUGU (JP)
Application Number:
PCT/JP2006/319192
Publication Date:
April 05, 2007
Filing Date:
September 27, 2006
Export Citation:
Assignee:
EISAI R&D MAN CO LTD (JP)
FUJIKAWA TOSHIHIKO (JP)
USUI MITSUGU (JP)
FUJIKAWA TOSHIHIKO (JP)
USUI MITSUGU (JP)
International Classes:
G01N33/53; C12N15/09; C12Q1/68; G01N21/64; G01N33/543
Domestic Patent References:
| WO2003029441A1 | 2003-04-10 | |||
| WO2002018642A1 | 2002-03-07 | |||
| WO2004074480A1 | 2004-09-02 | |||
| WO2001075157A1 | 2001-10-11 |
Foreign References:
| JP2002501184A | 2002-01-15 | |||
| JP2002506984A | 2002-03-05 | |||
| JP2005091014A | 2005-04-07 | |||
| JP2003088390A | 2003-03-25 | |||
| JP2002501184A | 2002-01-15 | |||
| JP3267576B2 | 2002-03-18 | |||
| EP1188841A1 | 2002-03-20 |
Other References:
See also references of EP 1939622A4
Attorney, Agent or Firm:
ISHIHARA, Shoji (No. 302 Wakai Bldg., 7-8, Higashi-Ikebukuro 3-chome, Toshima-k, Tokyo 13, JP)
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