Title:
METHOD FOR FORMING CONCENTRATION GRADIENT ON MICROREACTOR CHIP, AND MICROREACTOR CHIP
Document Type and Number:
WIPO Patent Application WO/2019/208795
Kind Code:
A1
Abstract:
A method for forming a concentration gradient on a microreactor chip comprises the steps of: introducing a first aqueous solution into a first liquid flow path arranged on the main surface of a hydrophobic layer of a microreactor chip provided with the hydrophobic layer, wherein the hydrophobic layer is made from a hydrophobic substance and has, formed on the main surface thereof, openings for multiple chambers, and the openings are arranged regularly on the main surface of the layer, whereby each of the chambers that face the first liquid flow path is filled with the first aqueous solution; introducing a second aqueous solution having a different concentration from that of the first aqueous solution through a first inlet port of the first liquid flow path to form a gradient in the concentration of an aqueous solution filled in each of the chambers, wherein the gradient corresponds to the distance from the first inlet port; and introducing an organic solvent containing a lipid and a third aqueous solution in this order into the first liquid flow path to form a lipid bilayer in such a manner that the opening for each of the chambers is sealed with an aqueous solution.
Inventors:
WATANABE RIKIYA (JP)
NOJI HIROYUKI (JP)
NOJI HIROYUKI (JP)
Application Number:
PCT/JP2019/018008
Publication Date:
October 31, 2019
Filing Date:
April 26, 2019
Export Citation:
Assignee:
UNIV TOKYO (JP)
International Classes:
C12M1/34; B01J19/00; C12M1/00; G01N35/02
Domestic Patent References:
| WO2018003856A1 | 2018-01-04 |
Foreign References:
| JP2015040754A | 2015-03-02 |
Other References:
SOGA, N. ET AL.: "Attolitre-sized lipid bilayer chamber array for rapid detection of single transporters", SCI REP., vol. 5, no. 1, 8 June 2015 (2015-06-08), pages 11025-1 - 11025-8, XP055646430
GE, S. ET AL.: "Digital, ultrasensitive, end-point protein measurements with large dynamic range via Brownian trapping with drift", J AM CHEM SOC., vol. 136, no. 42, 22 October 2014 (2014-10-22), pages 14662 - 5, XP055646430
GUERMONPREZ, C. ET AL.: "Flow distribution in parallel microfluidic networks and its effect on concentration gradient", BIOMICROFLUIDICS, vol. 9, no. 5, 6 October 2015 (2015-10-06), pages 054119-1 - 054119-13, XP055647031
HOLDEN, M. A. ET AL.: "Generating fixed concentration arrays in a microfluidic device", SENSORS AND ACTUATORS B, vol. 92, no. 1 , 2, 1 July 2003 (2003-07-01), pages 199 - 207, XP004424478, DOI: 10.1016/S0925-4005(03)00129-1
WATANABE, R. ET AL.: "High-throughput single- molecule bioassay using micro-reactor arrays with a concentration gradient of target molecules", LAB CHIP., vol. 18, no. 18, 11 September 2018 (2018-09-11), pages 2849 - 2853, XP055647037
GE, S. ET AL.: "Digital, ultrasensitive, end-point protein measurements with large dynamic range via Brownian trapping with drift", J AM CHEM SOC., vol. 136, no. 42, 22 October 2014 (2014-10-22), pages 14662 - 5, XP055646430
GUERMONPREZ, C. ET AL.: "Flow distribution in parallel microfluidic networks and its effect on concentration gradient", BIOMICROFLUIDICS, vol. 9, no. 5, 6 October 2015 (2015-10-06), pages 054119-1 - 054119-13, XP055647031
HOLDEN, M. A. ET AL.: "Generating fixed concentration arrays in a microfluidic device", SENSORS AND ACTUATORS B, vol. 92, no. 1 , 2, 1 July 2003 (2003-07-01), pages 199 - 207, XP004424478, DOI: 10.1016/S0925-4005(03)00129-1
WATANABE, R. ET AL.: "High-throughput single- molecule bioassay using micro-reactor arrays with a concentration gradient of target molecules", LAB CHIP., vol. 18, no. 18, 11 September 2018 (2018-09-11), pages 2849 - 2853, XP055647037
Attorney, Agent or Firm:
OHNO Seiji et al. (JP)
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