Title:
METHOD FOR HETEROLOGOUS EXPRESSION OF EPOTHILONE
Document Type and Number:
WIPO Patent Application WO/2018/149282
Kind Code:
A1
Abstract:
Disclosed is a method for heterologous expression of an epothilone, comprising introducing an epothilone gene cluster into host bacteria, while supplementing an epothilone precursor synthesis pathway combining with the introduction of the related tRNA gene and insertion of a promoter. The method can substantially increase the expression quantity of the epothilone, and the yield can be improved by about four orders of magnitude and can reach 8.5 mg/L. In addition, also provided are a genetic engineering strain for heterologous expression of the epothilone and a method for producing the epothilone.
Inventors:
DING XIAOMING (CN)
YU YUCONG (CN)
TANG BIAO (CN)
ZHANG LIN (CN)
WANG HUIMIN (CN)
BIAN XIAOYING (CN)
LI YUEZHONG (CN)
ZHANG YOUMING (CN)
ZHAO GUOPING (CN)
YU YUCONG (CN)
TANG BIAO (CN)
ZHANG LIN (CN)
WANG HUIMIN (CN)
BIAN XIAOYING (CN)
LI YUEZHONG (CN)
ZHANG YOUMING (CN)
ZHAO GUOPING (CN)
Application Number:
PCT/CN2018/074229
Publication Date:
August 23, 2018
Filing Date:
January 26, 2018
Export Citation:
Assignee:
UNIV FUDAN (CN)
International Classes:
C12N1/21; C12P17/18
Foreign References:
| CN1511192A | 2004-07-07 | |||
| CN1444655A | 2003-09-24 |
Other References:
BIAN, X. Y. ET AL.: "Heterologous Production and Yield Improvement of Epothilones in Burkholderiales Strain DSM 7029", ACS CHEMICAL BIOLOGY, vol. 12, no. 7, 3 May 2017 (2017-05-03), XP055535763
ZHOU, XI.: "Study on Fermentation Conditions of Epothilone-engineering Bacteria", CHINESE MASTER'S THESES FULL-TEXT DATABASE (BASIC SCIENCES), 15 March 2015 (2015-03-15)
ZHOU, XI.: "Study on Fermentation Conditions of Epothilone-engineering Bacteria", CHINESE MASTER'S THESES FULL-TEXT DATABASE (BASIC SCIENCES), 15 March 2015 (2015-03-15)
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