Title:
METHOD FOR HIGH-THROUGHPUT ANALYZING PROTEINS AND APPLICABLE LIBRARY THEREOF
Document Type and Number:
WIPO Patent Application WO/2019/149040
Kind Code:
A1
Abstract:
Disclosed are a method for high-throughput analyzing proteins and an applicable library thereof. The method for analyzing throughput proteins comprises using a tagged semi-cloned mouse library and conducting a parallel indication analysis for multiple different target proteins of interest with one or more tag protein antibodies. In the tagged semi-cloned mouse library, each semi-cloned mouse is a semi-cloned mouse or a sexually propagated progeny thereof obtained by injecting an ovum into a single male haploid embryonic stem cell and then cultivating, the single male haploid embryonic stem cell comprises the gene expressing the fusion protein of the target protein of interest and the tag protein, and the semi-cloned mice can express the fusion protein of the target protein of interest and the tag protein. The method provides a system suitable for high-throughput in vivo, real-time and dynamic research for the study of biomacromolecules.
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Inventors:
LI JINSONG (CN)
JIANG JING (CN)
BAI MEIZHU (CN)
YAN MENG (CN)
KANG JUNYAN (CN)
LI LIN (CN)
LI DANGSHENG (CN)
JIANG JING (CN)
BAI MEIZHU (CN)
YAN MENG (CN)
KANG JUNYAN (CN)
LI LIN (CN)
LI DANGSHENG (CN)
Application Number:
PCT/CN2019/071005
Publication Date:
August 08, 2019
Filing Date:
January 09, 2019
Export Citation:
Assignee:
SHANGHAI INST BIOLOGICAL SCIENCES CAS (CN)
International Classes:
G01N33/68; C12N5/0735; C12N15/85; C12Q1/68
Domestic Patent References:
| WO2017000302A1 | 2017-01-05 | |||
| WO2017000302A1 | 2017-01-05 |
Foreign References:
| CN106399311A | 2017-02-15 | |||
| CN106676075A | 2017-05-17 | |||
| CN103361304A | 2013-10-23 |
Other References:
YAN, MENG ET AL.: "Artificial Spermatids Derived Semi-clone Technology: From Origin to Application", CHINSES JOURNAL OF NATURE, vol. 40, no. 4, 31 August 2018 (2018-08-31), pages 245 - 252, XP009522029, ISSN: 0253-9608
JIANG, J. ET AL.: "Genome tagging project: tag every protein in mice through 'artificial spermatids", NATIONAL SCIENCE REVIEW, vol. 6, no. 3, 13 November 2018 (2018-11-13), pages 394 - 396, XP055629094
ZHONG, CUIQING ET AL.: "Artificial Sperms and CRISPR-Cas9", CHINESE BULLETIN OF LIFE SCIENCES, vol. 27, no. 12, 31 December 2015 (2015-12-31), pages 1489 - 1493, XP009522031, DOI: 10.13376/j.cbls/2015208
CHEN MOUTONG, LIU JIANJUN: "Research Techniques for Protein-protein Interactions", BIOTECHNOLOGY BULLETIN, no. 1, 31 December 2009 (2009-12-31), pages 50 - 54+ 68, XP009522032, ISSN: 1002-5464
See also references of EP 3748363A4
YANG, H.SHI, L.WANG, B.A.LIANG, D.ZHONG, C.LIU, W.NIE, Y.LIU, J.ZHAO, J.GAO, X. ET AL.: "Generation of genetically modified mice by oocyte injection of androgenetic haploid embryonic stem cells", CELL, vol. 149, 2012, pages 605 - 617, XP028420454, DOI: 10.1016/j.cell.2012.04.002
JIANG, J. ET AL.: "Genome tagging project: tag every protein in mice through 'artificial spermatids", NATIONAL SCIENCE REVIEW, vol. 6, no. 3, 13 November 2018 (2018-11-13), pages 394 - 396, XP055629094
ZHONG, CUIQING ET AL.: "Artificial Sperms and CRISPR-Cas9", CHINESE BULLETIN OF LIFE SCIENCES, vol. 27, no. 12, 31 December 2015 (2015-12-31), pages 1489 - 1493, XP009522031, DOI: 10.13376/j.cbls/2015208
CHEN MOUTONG, LIU JIANJUN: "Research Techniques for Protein-protein Interactions", BIOTECHNOLOGY BULLETIN, no. 1, 31 December 2009 (2009-12-31), pages 50 - 54+ 68, XP009522032, ISSN: 1002-5464
See also references of EP 3748363A4
YANG, H.SHI, L.WANG, B.A.LIANG, D.ZHONG, C.LIU, W.NIE, Y.LIU, J.ZHAO, J.GAO, X. ET AL.: "Generation of genetically modified mice by oocyte injection of androgenetic haploid embryonic stem cells", CELL, vol. 149, 2012, pages 605 - 617, XP028420454, DOI: 10.1016/j.cell.2012.04.002
Attorney, Agent or Firm:
J.Z.M.C. PATENT AND TRADEMARK LAW OFFICE (GENERAL PARTNERSHIP) (CN)
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