METHOD FOR HIGHLY EFFICIENTLY PRODUCING HETEROLOGOUS PROTEIN - NAT INST FOR AGRO ENVIRONMENTAL SCIENCES

Title:

METHOD FOR HIGHLY EFFICIENTLY PRODUCING HETEROLOGOUS PROTEIN

Document Type and Number:

WIPO Patent Application WO/2014/109360

Kind Code:

A1

Abstract:

Provided is a method which comprises developing a high-expression promoter of Pseudozyma antarctica, constructing a heterologous protein production system with the use of the obtained high-expression promoter, and then efficiently producing an enzyme using a strain belonging to the order Ustilaginales including a yeast of the genus Pseudozyma. The heterologous protein production method according to the present invention comprises: a step for culturing host cells, which have been transformed with an expression vector that contains a base sequence represented by SEQ ID NO:1 or SEQ ID NO:7 and a base sequence, said base sequence encoding a heterologous protein, functionally bound thereto, under such conditions as allowing the expression of the heterologous protein; and a step for harvesting the heterologous protein from a liquid culture medium.

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Inventors:

KITAMOTO HIROKO (JP)
WATANABE TAKASHI (JP)
MORITA TOMOTAKE (JP)
KOITABASHI MOTOO (JP)
YOSHIDA SHIGENOBU (JP)

Application Number:

PCT/JP2014/050233

Publication Date:

July 17, 2014

Filing Date:

January 09, 2014

Export Citation:

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Assignee:

NAT INST FOR AGRO ENVIRONMENTAL SCIENCES (JP)
NAT INST OF ADVANCED IND SCIEN (JP)

International Classes:

C12N15/09; C12N1/19; C12N9/16; C12N15/11; C12P21/02

Domestic Patent References:

WO2012092676A1

2012-07-12

Foreign References:

JP2009124995A	2009-06-11
JP2008237212A	2008-10-09
JP2010099066A	2010-05-06

Other References:

KIMBERLY G. NUGENT ET AL.: "Gene expression during Ustilago maydis diploid filamentous growth: EST library creation and analyses", FUNGAL GENETICS AND BIOLOGY, vol. 41, 2004, pages 349 - 360
DURIYA CHANTASINGH ET AL.: "Cloning, expression, and characterization of a xylanase 10 from Aspergillus terreus (BCC129) in Pichia pastoris", PROTEIN EXPRESSION AND PURIFICATION, vol. 46, 2006, pages 143 - 149
XIAOXIAO GUO ET AL.: "A novel pathway construction in Candida tropicalis for direct xylitol conversion from corncob xylan", BIORESOURCE TECHNOLOGY, vol. 128, 7 November 2012 (2012-11-07), pages 547 - 552
YALI CHENG ET AL.: "Recombinant Protein Secretion in Pseudozyma flocculosa and Pseudozyma antarctica with a Novel Signal Peptide", BIOSCIENCE, BIOTECHNOLOGY, AND BIOCHEMISTRY, vol. 72, no. 12, 2008, pages 3158 - 3166
KAZUO MASAKI ET AL.: "Construction of a new recombinant protein expression system in the basidiomycetous yeast Cryptococcus sp. strain S-2 and enhancement of the production of a cutinase-like enzyme", APPLIED GENETICS AND MOLECULAR BIOTECHNOLOGY, vol. 93, 15 November 2011 (2011-11-15), pages 1627 - 1636
T. J. AVIS ET AL.: "The potential of Pseudozyma yeastlike epiphytes for the production of heterologous recombinant proteins", APPLIED GENETICS AND MOLECULAR BIOTECHNOLOGY, vol. 69, 14 April 2005 (2005-04-14), pages 304 - 311
BERTRAND NEVEU ET AL.: "The Pseudozyma flocculosa actin promoter allows the strong expression of a recombinant protein in the Pseudozyma species", APPLIED GENETICS AND MOLECULAR BIOTECHNOLOGY, vol. 74, 6 January 2007 (2007-01-06), pages 1300 - 1307