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Title:
METHOD FOR IDENTIFYING BIOACTIVE PROTEIN, AND BIOACTIVE PROTEIN OBTAINED BY SAID METHOD
Document Type and Number:
WIPO Patent Application WO/2017/047672
Kind Code:
A1
Abstract:
The purpose of the present invention is to provide a method for identifying a bioactive protein translated from an open reading frame (ORF) other than a mORF present in mRNA. This problem is solved by a method for identifying a bioactive protein that is characterized by identifying a bioactive protein-encoding ORF other than the main ORF in the mRNA of a eukaryote, said method including: (1) a step in which an expression vector containing a candidate ORF is introduced into a cell, and the vector-introduced cell is cultured; (2) a step in which a protein that binds to a candidate protein, which is translated from the candidate ORF, is detected by immunoprecipitating the candidate protein from the cultured cell; and (3) a step in which the candidate protein, for which another protein that binds to the candidate protein was detected, is determined to be a bioactive protein.

Inventors:
AIZAWA Yasunori (2-12-1 Ookayama, Meguro-k, Tokyo 50, 〒1528550, JP)
KITANO Shohei (2-12-1 Ookayama, Meguro-k, Tokyo 50, 〒1528550, JP)
KIDA Yuichiro (4-8 Minamikibogaoka, Asahi-ku, Yokohama-sh, Kanagawa 24, 〒2410824, JP)
Application Number:
JP2016/077198
Publication Date:
March 23, 2017
Filing Date:
September 14, 2016
Export Citation:
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Assignee:
TOKYO INSTITUTE OF TECHNOLOGY (2-12-1, Ookayama Meguro-k, Tokyo 50, 〒1528550, JP)
LIFEIS INC. (4-8 Minamikibogaoka, Asahi-ku Yokohama-sh, Kanagawa 24, 〒2410824, JP)
International Classes:
C12N15/09; A01K67/027; C07K14/47; C07K14/575; C07K16/26; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12Q1/02; C12Q1/68; C12P21/08
Foreign References:
JP2010022261A2010-02-04
EP1347048A12003-09-24
Other References:
FUKUDA MAKIHA. ET AL.: "Hypothetical gene C18orf42 encodes a novel protein kinase A- binding protein", GENES TO CELLS, vol. 20, 4 February 2015 (2015-02-04), pages 267 - 280
VATTEM K M. ET AL.: "Reinitiation involving upstream ORFs regulates ATF4 mRNA translation in mammalian cells", PNAS, vol. 101, 2004, pages 11269 - 11274, XP055369009
Attorney, Agent or Firm:
MORITA Kenichi et al. (5th Fl, Itabashi-chuo Bldg. 67-8, Itabashi 2-chome, Itabashi-k, Tokyo 04, 〒1730004, JP)
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