Title:
METHOD OF IDENTIFYING HAIRPIN DNA PROBES BY PARTIAL FOLD ANALYSIS
Document Type and Number:
WIPO Patent Application WO2005104813
Kind Code:
A3
Abstract:
Method of identifying molecular beacons in which a secondary structure prediction algorithm is employed to identify oligonucleotide sequences within a target gene having the requisite hairpin structure. Isolated oligonucleotides, molecular beacons prepared from those oligonucleotides, and their use are also disclosed.
Inventors:
MILLER BENJAMIN L (US)
STROHSAHL CHRISTOPHER M (US)
STROHSAHL CHRISTOPHER M (US)
Application Number:
PCT/US2005/000053
Publication Date:
September 14, 2006
Filing Date:
January 03, 2005
Export Citation:
Assignee:
UNIV ROCHESTER (US)
MILLER BENJAMIN L (US)
STROHSAHL CHRISTOPHER M (US)
MILLER BENJAMIN L (US)
STROHSAHL CHRISTOPHER M (US)
International Classes:
C12Q1/68; C07H21/02; C07H21/04
Foreign References:
| US6355437B1 | 2002-03-12 |
Other References:
BONNET G. ET AL: "Thermodynamic basis of the enhanced specificity of structured DNA probes", PROC. NATL. ACAD. SCI. USA, vol. 96, May 1999 (1999-05-01), pages 6171 - 6176, XP002244958
DU H. ET AL: "Hybridization-Based Unquenching of DNA Hairpins on Au Surfaces: Prototypical "Molecular Beacon" Biosensors", J. AM. CHEM. SOC., vol. 125, March 2003 (2003-03-01), pages 4012 - 4013, XP002307240
DUBERTRET B. ET AL: "Single-mismatch detection using gold-quenched fluorescent oligonucleotides", NATURE BIOTECHNOLOGY, vol. 19, April 2001 (2001-04-01), pages 365 - 370, XP002224627
DU H. ET AL: "Hybridization-Based Unquenching of DNA Hairpins on Au Surfaces: Prototypical "Molecular Beacon" Biosensors", J. AM. CHEM. SOC., vol. 125, March 2003 (2003-03-01), pages 4012 - 4013, XP002307240
DUBERTRET B. ET AL: "Single-mismatch detection using gold-quenched fluorescent oligonucleotides", NATURE BIOTECHNOLOGY, vol. 19, April 2001 (2001-04-01), pages 365 - 370, XP002224627
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