Title:
METHOD FOR IDENTIFYING HIGH-AFFINITY COMPLEXES OF TWO LIGANDS AND A RECEPTOR, DEVICE FOR CARRYING OUT THE METHOD AND SELF-ASSEMBLYING CHEMICAL LIBRARY FOR USE WITH SAID METHOD
Document Type and Number:
WIPO Patent Application WO/2016/008822
Kind Code:
A3
Abstract:
The present invention relates to a method for the sensitive identification of high-affinity complexes of two ligands (2, 3, 4, 5, 6, 7) and a receptor (1). To this end, a plurality of different ligands (2, 3, 4, 5, 6, 7) of a chemical library are brought into contact with at least one receptor (1) in a solution. The ligands of the library have single-stranded DNA (8, 9) or RNA with a base length of 2 to 10 bases or, alternatively, more than 10 bases. The solution is incubated for a specified period of time and complexes of two ligands (2, 3, 4, 5, 6, 7) and a receptor (1) are identified.
Inventors:
REDDAVIDE FRANCESCO (DE)
DE ANDRADE HELENA (DE)
LIN WEILIN (DE)
ZHANG YIXIN (DE)
MELE ELISA (IT)
DE ANDRADE HELENA (DE)
LIN WEILIN (DE)
ZHANG YIXIN (DE)
MELE ELISA (IT)
Application Number:
PCT/EP2015/065874
Publication Date:
May 19, 2016
Filing Date:
July 10, 2015
Export Citation:
Assignee:
UNIV DRESDEN TECH (DE)
International Classes:
C40B30/04; B01L3/00; B01L7/00; C12Q1/68; G01N33/53
Domestic Patent References:
| WO2003076943A1 | 2003-09-18 | |||
| WO2010150103A2 | 2010-12-29 | |||
| WO2002081729A2 | 2002-10-17 | |||
| WO1997043232A1 | 1997-11-20 | |||
| WO2014005969A1 | 2014-01-09 |
Foreign References:
| US20100159455A1 | 2010-06-24 | |||
| US20110086361A1 | 2011-04-14 | |||
| US20110111968A1 | 2011-05-12 | |||
| US20100209925A1 | 2010-08-19 |
Other References:
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CIOBANU M ET AL: "Selection of a synthetic glycan oligomer from a library of DNA-templated fragments against DC-SIGN and inhibition of HIV gp120 binding to dendritic cells", CHEMICAL COMMUNICATIONS, vol. 47, no. 33, 25 July 2011 (2011-07-25), pages 9321, XP055212502, ISSN: 1359-7345, DOI: 10.1039/c1cc13213j
FRANZINI RM ET AL: "DNA-Encoded Chemical Libraries: Advancing beyond Conventional Small-Molecule Libraries", ACCOUNTS OF CHEMICAL RESEARCH, vol. 47, no. 4, 28 March 2014 (2014-03-28), pages 1247 - 1255, XP055211954, ISSN: 0001-4842, DOI: 10.1021/ar400284t
MANNOCCI L ET AL: "20 years of DNA-encoded chemical libraries", CHEMICAL COMMUNICATIONS > CHEMICAL COMMUNICATIONS - CHEMCOM; [6015D], ROYAL SOCIETY OF CHEMISTRY, GB, vol. 47, no. 48, 28 December 2011 (2011-12-28), pages 12747 - 12753, XP002695069, ISSN: 1359-7345, [retrieved on 20111114], DOI: 10.1039/C1CC15634A
BULLER F ET AL: "Drug discovery with DNA-encoded chemical libraries", BIOCONJUGATE CHEMISTRY, ACS, WASHINGTON, DC, US, vol. 21, no. 9, 15 September 2010 (2010-09-15), pages 1571 - 1580, XP002695068, ISSN: 1043-1802, [retrieved on 20100803], DOI: 10.1021/BC1001483
SCHEUERMANN J ET AL: "DNA-encoded chemical libraries for the discovery of MMP-3 inhibitors", BIOCONJUGATE CHEMISTRY, ACS, WASHINGTON, DC, US, vol. 19, no. 3, 1 March 2008 (2008-03-01), pages 778 - 785, XP002737640, ISSN: 1043-1802, [retrieved on 20080207], DOI: 10.1021/BC7004347
SCHEUERMANN J ET AL: "Dual-pharmacophore DNA-encoded chemical libraries", CURRENT OPINION IN CHEMICAL BIOLOGY, vol. 26, 13 March 2015 (2015-03-13), pages 99 - 103, XP055211951, ISSN: 1367-5931, DOI: 10.1016/j.cbpa.2015.02.021
CIOBANU M ET AL: "Selection of a synthetic glycan oligomer from a library of DNA-templated fragments against DC-SIGN and inhibition of HIV gp120 binding to dendritic cells", CHEMICAL COMMUNICATIONS, vol. 47, no. 33, 25 July 2011 (2011-07-25), pages 9321, XP055212502, ISSN: 1359-7345, DOI: 10.1039/c1cc13213j
FRANZINI RM ET AL: "DNA-Encoded Chemical Libraries: Advancing beyond Conventional Small-Molecule Libraries", ACCOUNTS OF CHEMICAL RESEARCH, vol. 47, no. 4, 28 March 2014 (2014-03-28), pages 1247 - 1255, XP055211954, ISSN: 0001-4842, DOI: 10.1021/ar400284t
MANNOCCI L ET AL: "20 years of DNA-encoded chemical libraries", CHEMICAL COMMUNICATIONS > CHEMICAL COMMUNICATIONS - CHEMCOM; [6015D], ROYAL SOCIETY OF CHEMISTRY, GB, vol. 47, no. 48, 28 December 2011 (2011-12-28), pages 12747 - 12753, XP002695069, ISSN: 1359-7345, [retrieved on 20111114], DOI: 10.1039/C1CC15634A
BULLER F ET AL: "Drug discovery with DNA-encoded chemical libraries", BIOCONJUGATE CHEMISTRY, ACS, WASHINGTON, DC, US, vol. 21, no. 9, 15 September 2010 (2010-09-15), pages 1571 - 1580, XP002695068, ISSN: 1043-1802, [retrieved on 20100803], DOI: 10.1021/BC1001483
SCHEUERMANN J ET AL: "DNA-encoded chemical libraries for the discovery of MMP-3 inhibitors", BIOCONJUGATE CHEMISTRY, ACS, WASHINGTON, DC, US, vol. 19, no. 3, 1 March 2008 (2008-03-01), pages 778 - 785, XP002737640, ISSN: 1043-1802, [retrieved on 20080207], DOI: 10.1021/BC7004347
SCHEUERMANN J ET AL: "Dual-pharmacophore DNA-encoded chemical libraries", CURRENT OPINION IN CHEMICAL BIOLOGY, vol. 26, 13 March 2015 (2015-03-13), pages 99 - 103, XP055211951, ISSN: 1367-5931, DOI: 10.1016/j.cbpa.2015.02.021
Attorney, Agent or Firm:
RIEPE, Hans-Gerd (Meinig & Partner mbBTheresienhöhe 11a, München, DE)
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