Title:
METHOD OF IDENTIFYING OR PRESUMING GENE UNDER REGULATION REGULATED BY FUNCTIONAL RNA AND METHOD OF USING THE SAME
Document Type and Number:
WIPO Patent Application WO/2005/017145
Kind Code:
A1
Abstract:
To control gene expression with the use of miRNAs via the phenomenon of gene expression regulation by miRNA, which is a gene regulation system occurring in nature, it is intended to presume or identify miRNA(s) and one or more genes under the regulation targeted thereby. Base sequences of miRNA molecules are divided into a region having a base sequence common to a plural number of miRNAs (a conserved region) and another region with uncommon base sequences (region other than the conserved region; unconserved region). Then ambiguities (searching accuracy levels) of different standards are set in these regions, that is, ambiguity of a relatively mild standard is set in the conserved region while ambiguity of a relatively strict standard is set in the unconserved region. Using the base sequences for searching thus obtained, a gene having a base sequence complementary to a functional RNA molecule (namely, a candidate for the gene under the regulation) is searched for followed by presumption or identification.
Inventors:
BARRERO ROBERTO ANTONIO (JP)
TAMURA TAKURO (JP)
IMANISHI TADASHI (JP)
GOJOBORI TAKASHI (JP)
TAIRA KAZUNARI (JP)
KAWASAKI HIROAKI (JP)
TAMURA TAKURO (JP)
IMANISHI TADASHI (JP)
GOJOBORI TAKASHI (JP)
TAIRA KAZUNARI (JP)
KAWASAKI HIROAKI (JP)
Application Number:
PCT/JP2004/011624
Publication Date:
February 24, 2005
Filing Date:
August 12, 2004
Export Citation:
Assignee:
JAPAN BIOLOG INFORMATICS CONSO (JP)
BITS CO LTD (JP)
NAT INST OF ADVANCED IND SCIEN (JP)
BARRERO ROBERTO ANTONIO (JP)
TAMURA TAKURO (JP)
IMANISHI TADASHI (JP)
GOJOBORI TAKASHI (JP)
TAIRA KAZUNARI (JP)
KAWASAKI HIROAKI (JP)
BITS CO LTD (JP)
NAT INST OF ADVANCED IND SCIEN (JP)
BARRERO ROBERTO ANTONIO (JP)
TAMURA TAKURO (JP)
IMANISHI TADASHI (JP)
GOJOBORI TAKASHI (JP)
TAIRA KAZUNARI (JP)
KAWASAKI HIROAKI (JP)
International Classes:
C12N15/11; (IPC1-7): C12N15/00; A61K31/711; C12N15/09
Domestic Patent References:
| WO2001062973A1 | 2001-08-30 |
Other References:
JENKINS Z A ET AL: "Human EIF5A2 on Chromosome 3q25-q27 Is a Phylogenetically Conserved Vertebrate Variant of Eukaryotic Translation Initiation Factor 5A with Tissue-Specific Expression", GENOMICS, vol. 71, 2001, pages 101 - 109, XP002904214
BINGLE C D ET AL: "Molecular cloning of the forkhead transcription factor HNF-3alpha from a human pulmonary adenocarcinoma cell line", BIOCHIMICA ET BIOPHYSICA ACTA, vol. 1307, 1996, pages 17 - 20, XP002904215
MARTINEZ L A ET AL: "Synthetic small inhibiting RNAS: Efficient tools to inactivate oncogenic mutations and restore p53 pathways", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, vol. 99, 2002, pages 14849 - 14854, XP002225999
RHOADES M W ET AL: "Prediction of plant microRNA targets", CELL, vol. 110, 23 August 2002 (2002-08-23), pages 513 - 520, XP002295225
BINGLE C D ET AL: "Molecular cloning of the forkhead transcription factor HNF-3alpha from a human pulmonary adenocarcinoma cell line", BIOCHIMICA ET BIOPHYSICA ACTA, vol. 1307, 1996, pages 17 - 20, XP002904215
MARTINEZ L A ET AL: "Synthetic small inhibiting RNAS: Efficient tools to inactivate oncogenic mutations and restore p53 pathways", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, vol. 99, 2002, pages 14849 - 14854, XP002225999
RHOADES M W ET AL: "Prediction of plant microRNA targets", CELL, vol. 110, 23 August 2002 (2002-08-23), pages 513 - 520, XP002295225
Attorney, Agent or Firm:
Hiraki, Yusuke (3-20 Toranomon 4-chom, Minato-ku Tokyo 01, JP)
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