Title:
METHOD OF IDENTIFYING A RESIN FOR ISOLATING OR ENRICHING A PROTEIN
Document Type and Number:
WIPO Patent Application WO/2020/053451
Kind Code:
A3
Abstract:
There is provided a method of identifying a resin for isolating or enriching a protein of interest using affinity chromatography. The method comprises the steps of: i) providing the three-dimensional structure of the protein of interest; ii) determining and/or calculating one or more parameters of the protein of interest in its two- and/or three-dimensional form; iii) determining and/or calculating one or more parameters of one or more resin in their two- and/or three-dimensional form; and iv) selecting a resin expected to bind complementarily to the protein of interest based upon one or more of the parameters of the protein of interest.
Inventors:
KNOX ANDREW (IE)
Application Number:
PCT/EP2019/074754
Publication Date:
April 30, 2020
Filing Date:
September 16, 2019
Export Citation:
Assignee:
UNIV DUBLIN TECHNOLOGICAL (IE)
International Classes:
G16B15/30; B01D15/08; B01J20/281; C07K1/22
Foreign References:
| US20070099235A1 | 2007-05-03 | |||
| US20130295646A1 | 2013-11-07 |
Other References:
INSAIDOO F K ET AL: "Targeted purification development enabled by computational biophysical modeling", BIOTECHNOLOGY PROGRESS, AMERICAN CHEMICAL SOCIETY, vol. 31, no. 1, 1 January 2015 (2015-01-01), pages 154 - 164, XP002757676, ISSN: 8756-7938, [retrieved on 20141219], DOI: 10.1002/BTPR.2023
YANG T ET AL: "Investigation of multi-modal high-salt binding ion-exchange chromatography using quantitative structure-property relationship modeling", JOURNAL OF CHROMATOGRAPHY A, ELSEVIER, AMSTERDAM, NL, vol. 1175, no. 1, 18 October 2007 (2007-10-18), pages 96 - 105, XP022354419, ISSN: 0021-9673, DOI: 10.1016/J.CHROMA.2007.10.037
MAHN A ET AL: "A theory of protein-resin interaction in hydrophobic interaction chromatography", JOURNAL OF CHROMATOGRAPHY A, ELSEVIER, AMSTERDAM, NL, vol. 1066, no. 1-2, 25 February 2005 (2005-02-25), pages 81 - 88, XP027722804, ISSN: 0021-9673, [retrieved on 20050225]
YANG T ET AL: "Investigation of multi-modal high-salt binding ion-exchange chromatography using quantitative structure-property relationship modeling", JOURNAL OF CHROMATOGRAPHY A, ELSEVIER, AMSTERDAM, NL, vol. 1175, no. 1, 18 October 2007 (2007-10-18), pages 96 - 105, XP022354419, ISSN: 0021-9673, DOI: 10.1016/J.CHROMA.2007.10.037
MAHN A ET AL: "A theory of protein-resin interaction in hydrophobic interaction chromatography", JOURNAL OF CHROMATOGRAPHY A, ELSEVIER, AMSTERDAM, NL, vol. 1066, no. 1-2, 25 February 2005 (2005-02-25), pages 81 - 88, XP027722804, ISSN: 0021-9673, [retrieved on 20050225]
Attorney, Agent or Firm:
BARKER BRETTELL LLP (GB)
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