Title:
METHOD FOR IDENTIFYING TARGET PROTEIN USING THERMAL STABILITY SHIFT-BASED FLUORESCENCE DIFFERENCE IN TWO-DIMENSIONAL GEL ELECTROPHORESIS
Document Type and Number:
WIPO Patent Application WO/2018/062726
Kind Code:
A1
Abstract:
The present invention relates to a method for identifying a target protein using a thermal stability shift-based fluorescence difference in two-dimensional gel electrophoresis, and more specifically, a method for identifying a protein, which is a target of a specific drug, by analyzing, by means of a fluorescence difference in two-dimensional gel electrophoresis, a thermal stability change in the protein when the specific drug, preferably a bioactive molecule, binds to the target protein.
Inventors:
PARK SEUNG BUM (KR)
PARK HAN KUM (KR)
PARK HAN KUM (KR)
Application Number:
PCT/KR2017/010044
Publication Date:
April 05, 2018
Filing Date:
September 13, 2017
Export Citation:
Assignee:
SPARK BIOPHARMA INC (KR)
International Classes:
G01N33/52; G01N33/58; G01N33/68
Foreign References:
| KR101427328B1 | 2014-08-06 | |||
| KR20140033366A | 2014-03-18 | |||
| KR100857592B1 | 2008-09-09 |
Other References:
PARK, HANKUM ET AL.: "Label-free Target Identification Using In-gel Fluorescence Difference via Thermal Stability Shift", CHEMICAL SCIENCE, vol. 8, no. 2, 22 September 2016 (2016-09-22), pages 1127 - 1133, XP055515309
PARK, JONG MIN ET AL.: "Discovery and Target Identification of an Antiproliferative Agent in Live Cells Using Fluorescence Difference in Two-Dimensional Gel Electrophoresis", ANGEWANDTE CHEMIE, vol. 51, no. 22, 4 April 2012 (2012-04-04), pages 5447 - 5451, XP055515325
See also references of EP 3521825A4
PARK, JONG MIN ET AL.: "Discovery and Target Identification of an Antiproliferative Agent in Live Cells Using Fluorescence Difference in Two-Dimensional Gel Electrophoresis", ANGEWANDTE CHEMIE, vol. 51, no. 22, 4 April 2012 (2012-04-04), pages 5447 - 5451, XP055515325
See also references of EP 3521825A4
Attorney, Agent or Firm:
CHOI, Eun Sun (KR)
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