Title:
METHOD FOR IMMOBILIZING ENZYME ON ELECTRODE FOR FUEL CELL, FUEL CELL, METHOD FOR MANUFACTURING FUEL CELL, ELECTRODE FOR FUEL CELL, AND METHOD FOR MANUFACTURING ELECTRODE FOR FUEL CELL
Document Type and Number:
WIPO Patent Application WO/2010/061822
Kind Code:
A1
Abstract:
Provided are an enzyme immobilizing method, a fuel cell and an electrode for the fuel cell which employ the enzyme immobilizing method, and a method for manufacturing the fuel cell and the electrode. The enzyme immobilizing method prevents reduction in enzyme activity when the enzyme is immobilized on the electrode, so as to make it possible to obtain a high catalyst current value. In the method for immobilizing an enzyme on the electrode used in the fuel cell, an enzyme variant with at least one amino acid residue being deleted, substituted, added, or inserted in a wild type amino acid sequence is used as the enzyme, and the enzyme variant increases in activity through heat treatment. The immobilization is performed within a temperature range which makes it possible to increase the activity of the enzyme variant.
Inventors:
KUMITA HIDEYUKI (JP)
NAKAGAWA TAKAAKI (JP)
SAKAI HIDEKI (JP)
KAKUTA MASAYA (JP)
TOKITA YUICHI (JP)
NAKAGAWA TAKAAKI (JP)
SAKAI HIDEKI (JP)
KAKUTA MASAYA (JP)
TOKITA YUICHI (JP)
Application Number:
PCT/JP2009/069803
Publication Date:
June 03, 2010
Filing Date:
November 24, 2009
Export Citation:
Assignee:
SONY CORP (JP)
KUMITA HIDEYUKI (JP)
NAKAGAWA TAKAAKI (JP)
SAKAI HIDEKI (JP)
KAKUTA MASAYA (JP)
TOKITA YUICHI (JP)
KUMITA HIDEYUKI (JP)
NAKAGAWA TAKAAKI (JP)
SAKAI HIDEKI (JP)
KAKUTA MASAYA (JP)
TOKITA YUICHI (JP)
International Classes:
H01M4/88; H01M4/90; H01M8/16; C12N9/06; C12N11/00; C12R1/645
Foreign References:
| JP2009158480A | 2009-07-16 | |||
| JP2008161178A | 2008-07-17 | |||
| JP2006127957A | 2006-05-18 | |||
| JP2007280944A | 2007-10-25 | |||
| JP2004089042A | 2004-03-25 | |||
| JP2007225444A | 2007-09-06 | |||
| JP2008237099A | 2008-10-09 |
Other References:
DMITRI IVNITSKI ET AL.: "Surface characterization and direct electrochemistry of redox copper centers of bilirubin oxidase from fungi Myrothecium verrucaria", BIOELECTROCHEMISTRY, vol. 74, May 2008 (2008-05-01), pages 101 - 110
YUJI KAMITAKA ET AL.: "Effects of axial ligand mutation of the type I copper site in bilirubin oxidase on direct electron transfer-type bioelectrocatalytic reduction of dioxygen", JOURNAL OF ELECTROANALYTICAL CHEMISTRY, vol. 601, 2007, pages 119 - 124
YUJI KAMITAKA ET AL.: "Bilirubin Oxidase Oyobi sono Hen'itai no Koso -Denkyokukan Chokusetsu Denshi Ido Hanno", REVIEW OF POLAROGRAPHY, vol. 50, no. 3, 2004, pages 293
ATSUSHI SHIMIZU ET AL.: "Myrothecium verrucaria Bilirubin Oxidase and Its Mutants for Potential Copper Ligands", BIOCHEMISTRY, vol. 38, 1999, pages 3034 - 3042
YUJI KAMITAKA ET AL.: "Effects of axial ligand mutation of the type I copper site in bilirubin oxidase on direct electron transfer-type bioelectrocatalytic reduction of dioxygen", JOURNAL OF ELECTROANALYTICAL CHEMISTRY, vol. 601, 2007, pages 119 - 124
YUJI KAMITAKA ET AL.: "Bilirubin Oxidase Oyobi sono Hen'itai no Koso -Denkyokukan Chokusetsu Denshi Ido Hanno", REVIEW OF POLAROGRAPHY, vol. 50, no. 3, 2004, pages 293
ATSUSHI SHIMIZU ET AL.: "Myrothecium verrucaria Bilirubin Oxidase and Its Mutants for Potential Copper Ligands", BIOCHEMISTRY, vol. 38, 1999, pages 3034 - 3042
Attorney, Agent or Firm:
FUJISHIMA Youichiro et al. (JP)
Yoichiro Fujishima (JP)
Yoichiro Fujishima (JP)
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