Title:
METHOD FOR IMMOBILIZING AND RELEASING MICROORGANISM
Document Type and Number:
WIPO Patent Application WO/2014/156736
Kind Code:
A1
Abstract:
The present invention addresses the problem of providing a more practical technique for immobilizing a microorganism using Acinetobacter sp. Tol 5-derived adhesive protein AtaA. Provided is a method for attaching and detaching a microorganism, said method comprising: (1) a step for contacting a microorganism, into which DNA encoding autotransporter adhesin derived from a microorganism belonging to the genus Acinetobacter has been transferred to impart non-specific adhesiveness thereto or enhance non-specific adhesiveness thereof, with a carrier at a high ionic strength and thus attaching the microorganism to the carrier; and (2) a step for washing the carrier at a low ionic strength and thus releasing the microorganism from the carrier.
Inventors:
HORI KATSUTOSHI (JP)
Application Number:
PCT/JP2014/056966
Publication Date:
October 02, 2014
Filing Date:
March 14, 2014
Export Citation:
Assignee:
UNIV NAGOYA NAT UNIV CORP (JP)
International Classes:
C12N11/00; C12N1/21; C12N15/09
Domestic Patent References:
| WO2009104281A1 | 2009-08-27 | |||
| WO2009104281A1 | 2009-08-27 |
Other References:
KATSUTOSHI HORI ET AL.: "Microbial adhesion to surfaces mediated by bacterial nanofibers", JOURNAL OF ENVIRONMENTAL BIOTECHNOLOGY, vol. 10, no. 1, 31 July 2010 (2010-07-31), pages 3 - 7, XP008180856
ISHIKAWA M. ET AL.: "AtaA, a new member of the trimeric autotransporter adhesins from Acinetobacter sp. Tol 5 mediating high adhesiveness to various abiotic surfaces", PLOS ONE, vol. 7, no. 11, 14 November 2012 (2012-11-14), pages E48830, XP055280640
KATSUTOSHI HORI: "From Control and Application of Microbial Adhesion and Biofilms into Interfacial Microbial Engineering", OLEOSCIENCE, vol. 12, no. 11, 2012, pages 19 - 24, XP008180853
YUKI OHARA ET AL.: "Secchakusei Nanofiber Tanpakushitsu AtaA o Mochiita Gram Insei Saikin no Koteika", ABSTRACTS OF 78TH ANNUAL MEETING OF THE SOCIETY OF CHEMICAL ENGINEERS, 17 February 2013 (2013-02-17), JAPAN, pages L105, XP008180873
SHOGO YOSHIMOTO ET AL.: "Bacterionanofiber Tanpakushitsu AtaA o Kaishita Acinetobacter sp. Tol5 no Fuchaku Tokusei", ABSTRACTS OF 78TH ANNUAL MEETING OF THE SOCIETY OF CHEMICAL ENGINEERS, 17 February 2013 (2013-02-17), JAPAN, pages L104, XP008180872
YUKI OHARA ET AL.: "Evaluation of the new microbial immobilization method using the highly adhesive nanofiber AtaA : esterase reaction by Acinetobacter sp. ADP1 as a model", ABSTRACTS OF THE 65TH ANNUAL MEETING OF THE SOCIETY FOR BIOTECHNOLOGY, 25 August 2013 (2013-08-25), JAPAN, pages 236, 3P-194
ISHIKAWA M. ET AL.: "Application of the adhesive bacterionanofiber AtaA to a novel microbial immobilization method for the production of indigo as a model chemical", BIOTECHNOL.BIOENG., vol. 1, no. 1, 11 January 2014 (2014-01-11), pages 16 - 24, XP055280645
SHOGO YOSHIMOTO ET AL.: "Secchakusei Saibo Hyoso Tanpakushitsu no Tokusei ni Motozuku Biseibutsu Koteika Gijutsu", THE SOCIETY OF CHEMICAL ENGINEERS, JAPAN 45TH AUTUMN MEETING KOEN YOSHISHU, 16 August 2013 (2013-08-16), pages 67, XP008180871
SHOGO YOSHIMOTO ET AL.: "Nanofiber Tanpakushitsu AtaA no Secchaku Tokusei ni Motozuku Biseibutsu no Kagyakuteki Koteikaho", THE SOCIETY OF CHEMICAL ENGINEERS, JAPAN DAI 79 NENKAI KOEN YOSHISHU, 18 February 2014 (2014-02-18), pages 319, XP008180876
JUNTER, G. A.; JOUENNE, T.: "Immobilized viable microbial cells: from the process to the proteome ... or the cart before the horse", BIOTECHNOL. ADV., vol. 22, no. 8, 2004, pages 633 - 658
QURESHI, N.; ANNOUS, B. A.; EZEJI, T. C.; KARCHER, P.; MADDOX, 1. S.: "Biofilm reactors for industrial bioconversion processes: employing potential of enhanced reaction rates", MICROB. CELL. FACT., vol. 4, 2005, pages 24
LI, X. Z.; WEBB, J. S.; KJELLEBERG, S.; ROSCHE, B.: "Enhanced benzaldehyde tolerance in Zymomonas mobilis biofilms and the potential ofbiofilm applications in fine-chemical production", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 72, no. 2, 2006, pages 1639 - 1644
GROSS, R.; HAUER, B.; OTTO, K.; SCHMID, A.: "Microbial biofilms: new catalysts for maximizing productivity of long-term biotransformations", BIOTECHNOLOGY AND BIOENGINEERING, vol. 98, no. 6, 2007, pages 1123 - 1134
LI, X. Z.; HAUER, B.; ROSCHE, B.: "Single-species microbial biofilm screening for industrial applications", APPL. MICROBIOL. BIOTECHNOL, vol. 76, no. 6, 2007, pages 1255 - 1262
ROSCHE, B.; LI, X. Z.; HAUER, B.; SCHMID, A.; BUEHLER, K.: "Microbial biofilms: a concept for industrial catalysis?", TRENDS BIOTECHNOL, vol. 27, no. 11, 2009, pages 636 - 43
HALAN, B.; BUEHLER, K.; SCHMID, A.: "Biofilms as living catalysts in continuous chemical syntheses", TRENDS BIOTECHNOL, vol. 30, no. 9, 2012, pages 453 - 65
CHENG, K. C.; DEMIRCI, A.; CATCHMARK, J. M.: "Advances in bio film reactors for production of value-added products", APPL MICROBIOL BIOTECHNOL, vol. 87, no. 2, 2010, pages 445 - 56
ISHIKAWA, M.; NAKATANI, H.; HORI, K.: "AtaA, a new member of the trimeric autotransporter adhesins from Acinetobacter sp. Tol 5 mediating high adhesiveness to various abiotic surfaces", PLOS ONE, vol. 7, no. 11, 2012, pages E48830
LINKE, D.; RIESS, T.; AUTENRIETH, 1. B.; LUPAS, A.; KEMPF, V. A.: "Trimeric autotransporter adhesins: variable structure, common function", TRENDS MICROBIOL., vol. 14, no. 6, 2006, pages 264 - 270
SHANE E. COTTER; NEERAJ K.SURANA; JOSEPH W. ST: "Geme III 2005. Trimeric autotransporters: a distinct subfamily of Autotransporter proteins", TRENDS IN MICROBIOLOGY, vol. 13, pages 199 - 205
ANDREAS ROGGENKAMP; NIKOLAUS ACKERMANN; CHRISTOPH A. JACOBI; KONRAD TRUELZSCH; HARALD HOFFMANN; JURGEN HEESEMANN: "Molecular analysis of transport and oligomerization of the Yersinia enterocolitica adhesin YadA", J BACTERIOL., vol. 185, 2003, pages 3735 - 3744
SAMBROOK, J ET AL.: "Molecular Cloning", 1989, COLD SPRING HARBOR LAB. PRESS
"A Compendium of Methods from Current Protocols in Molecular Biology, Third Edition,", JOHN WILEY & SONS, INC, article "Short Protocols In Molecular Biology"
SIMON, R.; PRIEFER, U.; PUHLER, A., BIO-TECHNOL, vol. 1, no. 9, 1983, pages 784 - 791
ISHIKAWA M. ET AL.: "AtaA, a new member of the trimeric autotransporter adhesins from Acinetobacter sp. Tol 5 mediating high adhesiveness to various abiotic surfaces", PLOS ONE, vol. 7, no. 11, 14 November 2012 (2012-11-14), pages E48830, XP055280640
KATSUTOSHI HORI: "From Control and Application of Microbial Adhesion and Biofilms into Interfacial Microbial Engineering", OLEOSCIENCE, vol. 12, no. 11, 2012, pages 19 - 24, XP008180853
YUKI OHARA ET AL.: "Secchakusei Nanofiber Tanpakushitsu AtaA o Mochiita Gram Insei Saikin no Koteika", ABSTRACTS OF 78TH ANNUAL MEETING OF THE SOCIETY OF CHEMICAL ENGINEERS, 17 February 2013 (2013-02-17), JAPAN, pages L105, XP008180873
SHOGO YOSHIMOTO ET AL.: "Bacterionanofiber Tanpakushitsu AtaA o Kaishita Acinetobacter sp. Tol5 no Fuchaku Tokusei", ABSTRACTS OF 78TH ANNUAL MEETING OF THE SOCIETY OF CHEMICAL ENGINEERS, 17 February 2013 (2013-02-17), JAPAN, pages L104, XP008180872
YUKI OHARA ET AL.: "Evaluation of the new microbial immobilization method using the highly adhesive nanofiber AtaA : esterase reaction by Acinetobacter sp. ADP1 as a model", ABSTRACTS OF THE 65TH ANNUAL MEETING OF THE SOCIETY FOR BIOTECHNOLOGY, 25 August 2013 (2013-08-25), JAPAN, pages 236, 3P-194
ISHIKAWA M. ET AL.: "Application of the adhesive bacterionanofiber AtaA to a novel microbial immobilization method for the production of indigo as a model chemical", BIOTECHNOL.BIOENG., vol. 1, no. 1, 11 January 2014 (2014-01-11), pages 16 - 24, XP055280645
SHOGO YOSHIMOTO ET AL.: "Secchakusei Saibo Hyoso Tanpakushitsu no Tokusei ni Motozuku Biseibutsu Koteika Gijutsu", THE SOCIETY OF CHEMICAL ENGINEERS, JAPAN 45TH AUTUMN MEETING KOEN YOSHISHU, 16 August 2013 (2013-08-16), pages 67, XP008180871
SHOGO YOSHIMOTO ET AL.: "Nanofiber Tanpakushitsu AtaA no Secchaku Tokusei ni Motozuku Biseibutsu no Kagyakuteki Koteikaho", THE SOCIETY OF CHEMICAL ENGINEERS, JAPAN DAI 79 NENKAI KOEN YOSHISHU, 18 February 2014 (2014-02-18), pages 319, XP008180876
JUNTER, G. A.; JOUENNE, T.: "Immobilized viable microbial cells: from the process to the proteome ... or the cart before the horse", BIOTECHNOL. ADV., vol. 22, no. 8, 2004, pages 633 - 658
QURESHI, N.; ANNOUS, B. A.; EZEJI, T. C.; KARCHER, P.; MADDOX, 1. S.: "Biofilm reactors for industrial bioconversion processes: employing potential of enhanced reaction rates", MICROB. CELL. FACT., vol. 4, 2005, pages 24
LI, X. Z.; WEBB, J. S.; KJELLEBERG, S.; ROSCHE, B.: "Enhanced benzaldehyde tolerance in Zymomonas mobilis biofilms and the potential ofbiofilm applications in fine-chemical production", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 72, no. 2, 2006, pages 1639 - 1644
GROSS, R.; HAUER, B.; OTTO, K.; SCHMID, A.: "Microbial biofilms: new catalysts for maximizing productivity of long-term biotransformations", BIOTECHNOLOGY AND BIOENGINEERING, vol. 98, no. 6, 2007, pages 1123 - 1134
LI, X. Z.; HAUER, B.; ROSCHE, B.: "Single-species microbial biofilm screening for industrial applications", APPL. MICROBIOL. BIOTECHNOL, vol. 76, no. 6, 2007, pages 1255 - 1262
ROSCHE, B.; LI, X. Z.; HAUER, B.; SCHMID, A.; BUEHLER, K.: "Microbial biofilms: a concept for industrial catalysis?", TRENDS BIOTECHNOL, vol. 27, no. 11, 2009, pages 636 - 43
HALAN, B.; BUEHLER, K.; SCHMID, A.: "Biofilms as living catalysts in continuous chemical syntheses", TRENDS BIOTECHNOL, vol. 30, no. 9, 2012, pages 453 - 65
CHENG, K. C.; DEMIRCI, A.; CATCHMARK, J. M.: "Advances in bio film reactors for production of value-added products", APPL MICROBIOL BIOTECHNOL, vol. 87, no. 2, 2010, pages 445 - 56
ISHIKAWA, M.; NAKATANI, H.; HORI, K.: "AtaA, a new member of the trimeric autotransporter adhesins from Acinetobacter sp. Tol 5 mediating high adhesiveness to various abiotic surfaces", PLOS ONE, vol. 7, no. 11, 2012, pages E48830
LINKE, D.; RIESS, T.; AUTENRIETH, 1. B.; LUPAS, A.; KEMPF, V. A.: "Trimeric autotransporter adhesins: variable structure, common function", TRENDS MICROBIOL., vol. 14, no. 6, 2006, pages 264 - 270
SHANE E. COTTER; NEERAJ K.SURANA; JOSEPH W. ST: "Geme III 2005. Trimeric autotransporters: a distinct subfamily of Autotransporter proteins", TRENDS IN MICROBIOLOGY, vol. 13, pages 199 - 205
ANDREAS ROGGENKAMP; NIKOLAUS ACKERMANN; CHRISTOPH A. JACOBI; KONRAD TRUELZSCH; HARALD HOFFMANN; JURGEN HEESEMANN: "Molecular analysis of transport and oligomerization of the Yersinia enterocolitica adhesin YadA", J BACTERIOL., vol. 185, 2003, pages 3735 - 3744
SAMBROOK, J ET AL.: "Molecular Cloning", 1989, COLD SPRING HARBOR LAB. PRESS
"A Compendium of Methods from Current Protocols in Molecular Biology, Third Edition,", JOHN WILEY & SONS, INC, article "Short Protocols In Molecular Biology"
SIMON, R.; PRIEFER, U.; PUHLER, A., BIO-TECHNOL, vol. 1, no. 9, 1983, pages 784 - 791
Attorney, Agent or Firm:
HAGINO Mikiharu (JP)
Kanji Hagino (JP)
Kanji Hagino (JP)
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