Title:
METHOD FOR IMMUNOLOGICALLY ASSAYING HEMOGLOBIN A1C IN SPECIMEN
Document Type and Number:
WIPO Patent Application WO/2014/112318
Kind Code:
A1
Abstract:
Disclosed is a means whereby hemoglobin A1c in a whole blood sample can be immunologically assayed without preliminarily treating the sample. According to the present invention, the immunoassay of hemoglobin A1c in a specimen comprises contacting latex particles with a whole blood specimen, said whole blood specimen having not been preliminarily treated, in a hypotonic solution, and then contacting hemoglobin A1c adsorbed on the
surface of the latex particles with anti-hemoglobin A1c antibody. The immunoassay is preferably carried out by the aggregation method. The hypotonic solution is, for example, a buffer solution containing a good buffer, which shows the maximum buffer capacity in the neutral to alkaline side, at a concentration of 0.02-0.2 mol/L.
Inventors:
TETSUMOTO TORU (JP)
HIRATA MINORU (JP)
HIRATA MINORU (JP)
Application Number:
PCT/JP2013/084905
Publication Date:
July 24, 2014
Filing Date:
December 26, 2013
Export Citation:
Assignee:
FUJIREBIO KK (JP)
International Classes:
G01N33/48; G01N33/53; G01N33/545
Foreign References:
| JPH0735752A | 1995-02-07 | |||
| JP2004059477A | 2004-02-26 | |||
| JP2001296292A | 2001-10-26 | |||
| JP2677753B2 | 1997-11-17 |
Other References:
JOURNAL OF THE JAPAN DIABETES SOCIETY, vol. 53, no. 6, 2010, pages 450
See also references of EP 2947458A4
See also references of EP 2947458A4
Attorney, Agent or Firm:
TANIGAWA AND PARTNERS, PATENT FIRM (JP)
Patent business corporation mountain stream international patent firm (JP)
Patent business corporation mountain stream international patent firm (JP)
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