Title:
METHOD FOR IMPLEMENTING ULTRA-SENSITIVE BRINK CONTROL OVER DELAYED ENZYMATIC REACTION ON BASIS OF DNA STRAND DISPLACEMENT
Document Type and Number:
WIPO Patent Application WO/2024/041137
Kind Code:
A1
Abstract:
A method for implementing ultra-sensitive brink control over a delayed enzymatic reaction on the basis of DNA strand displacement, which method relates to the technical field of feedback control based on DNA strand displacement in biological systems. The method comprises: obtaining an enzymatic reaction process model, which has a time delay; constructing a brink controller based on CRNs; acquiring a static mapping expression between the output of the brink controller and the output of a system under a steady-state condition, so as to obtain an analysis condition capable of ensuring the performance of the controller; implementing the construction of the brink controller by means of a DNA strand displacement reaction; and acquiring a time delay representation mode, applying same to DNA implementation of the enzymatic reaction process model, and controlling the rewritten enzymatic reaction process model in view of the constructed brink controller. The method does not involve a subtraction operation in terms of structure, thereby reducing the number of abstract chemical reactions for implementation, and greatly simplifying the complexity of DNA implementation.
Inventors:
ZHANG QIANG (CN)
LV HUI (CN)
ZHOU SHIHUA (CN)
WANG BIN (CN)
XIAO YIJUN (CN)
WANG XINGAN (CN)
LV HUI (CN)
ZHOU SHIHUA (CN)
WANG BIN (CN)
XIAO YIJUN (CN)
WANG XINGAN (CN)
Application Number:
PCT/CN2023/101281
Publication Date:
February 29, 2024
Filing Date:
June 20, 2023
Export Citation:
Assignee:
UNIV DALIAN (CN)
International Classes:
G05B13/04; G16B5/00; G16C20/10
Foreign References:
| CN115407655A | 2022-11-29 | |||
| CN108009395A | 2018-05-08 | |||
| CN108596340A | 2018-09-28 | |||
| CN112348178A | 2021-02-09 | |||
| CN114428461A | 2022-05-03 | |||
| US7158888B2 | 2007-01-02 |
Other References:
ZHANG XIN-JIAN, KOU ZHENG: "Logical realization of molecular encoder based on DNA computing", JOURNAL OF GUANGZHOU UNIVERSITY(NATURAL SCIENCE EDITION)., vol. 19, no. 5, 1 October 2020 (2020-10-01), XP093142332, DOI: 1671-4229(2020)05-0012-06
SONG JIAYI, SU PING, YANG YE, YANG YI: "Preparation and application of a novel immobilization enzyme reactor based on DNA strand displacement reactions", SEPU - CHINESE JOURNAL OF CHROMATOGRAPHY, HUAXUE ZAZHI CHUBANSHE, DALIAN, CN, vol. 35, no. 3, 1 January 2017 (2017-01-01), CN , pages 260 - 263, XP093142335, ISSN: 1000-8713, DOI: 10.3724/SP.J.1123.2016.10023
SONG JIAYI, SU PING, YANG YE, YANG YI: "Preparation and application of a novel immobilization enzyme reactor based on DNA strand displacement reactions", SEPU - CHINESE JOURNAL OF CHROMATOGRAPHY, HUAXUE ZAZHI CHUBANSHE, DALIAN, CN, vol. 35, no. 3, 1 January 2017 (2017-01-01), CN , pages 260 - 263, XP093142335, ISSN: 1000-8713, DOI: 10.3724/SP.J.1123.2016.10023
Attorney, Agent or Firm:
BEIJING GAOWO LAW FIRM (CN)
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