Title:
METHOD OF IMPROVING BIOMASS YIELD OF LACTIC ACID BACTERIAL CULTURES
Document Type and Number:
WIPO Patent Application WO2002002747
Kind Code:
A3
Abstract:
A method of enhancing biomass yield of a lactic acid bacterial species cell culture, comprising cultivating the cells in a process comprising the steps of providing conditions that results in a reduced glycolytic flux and providing conditions that enable the cells to have, under aerobic conditions, a respiratory metabolism. The increased yield of biomass may be the result of an increased yield of ATP which can be obtained by activating the native ATP synthase activity of the H<+>-ATPase complex by lowering the ATP/ADP ratio, e.g. by carbon source limitation, and/or by increasing the proton gradient (membrane potential) of the cells, e.g. by enhancing or establishing an electron transport chain which can be achieved by enhancing expression of dehydrogenases or electron transport chain components, by adding to the medium a quinone or porphyrin compound or by enhancing the expression of the H<+>-ATPase activity.
Inventors:
JENSEN PETER RUHDAL (DK)
BLANK LARS (DE)
KOEBMANN BRIAN JENSEN (DK)
BLANK LARS (DE)
KOEBMANN BRIAN JENSEN (DK)
Application Number:
PCT/DK2001/000468
Publication Date:
May 10, 2002
Filing Date:
July 05, 2001
Export Citation:
Assignee:
UNIV DANMARKS TEKNISKE (DK)
JENSEN PETER RUHDAL (DK)
BLANK LARS (DE)
KOEBMANN BRIAN JENSEN (DK)
JENSEN PETER RUHDAL (DK)
BLANK LARS (DE)
KOEBMANN BRIAN JENSEN (DK)
International Classes:
C12N1/20; (IPC1-7): C12N1/20; C12N1/04
Domestic Patent References:
| WO1998010089A1 | 1998-03-12 | |||
| WO2000005342A1 | 2000-02-03 | |||
| WO1987003006A1 | 1987-05-21 |
Other References:
SALOU P ET AL: "Growth and energetics of leuconostoc oenos during cometabolism of glucose with citrate or fructose.", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 60, no. 5, May 1994 (1994-05-01), pages 1459 - 1466, XP002902323
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