Title:
METHOD FOR IMPROVING SAM COFACTOR SUPPLY OF SACCHAROMYCES CEREVISIAE, ENGINEERED YEAST AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2023/108505
Kind Code:
A1
Abstract:
Provided in the present application are a method for improving the SAM cofactor supply of Saccharomyces cerevisiae, an engineered yeast and the use thereof. The method comprises: introducing an MET6 gene, a cMTFHR gene, a LiMETK1 gene, an SAH1 gene and an ADO1 gene into Saccharomyces cerevisiae. The method can solve the problem of the insufficient supply of endogenous auxiliary factors in the synthesis process of methylation products such as ferulic acid, thereby increasing the synthesis efficiency thereof.
Inventors:
ZHOU YONGJIN (CN)
ZHANG LEI (CN)
CHEN RUIBING (CN)
ZHANG LEI (CN)
CHEN RUIBING (CN)
Application Number:
PCT/CN2021/138511
Publication Date:
June 22, 2023
Filing Date:
December 15, 2021
Export Citation:
Assignee:
DALIAN INST CHEM & PHYSICS CAS (CN)
International Classes:
C12N15/67; C12N1/19; C12N15/81; C12R1/865
Domestic Patent References:
| WO2006045953A1 | 2006-05-04 |
Foreign References:
| CN104818300A | 2015-08-05 |
Other References:
WANG, MENG ET AL.: "Cofactor engineering for more efficient production of chemicals and biofuels", BIOTECHNOLOGY ADVANCES, vol. 35, no. 8, 20 September 2017 (2017-09-20), XP085293645, DOI: 10.1016/j.biotechadv.2017.09.008
CHEN YAWEI, XU DUANBIN, FAN LIHAI, ZHANG XU, TAN TIANWEI: "Manipulating multi-system of NADPH regulation in Escherichia coli for enhanced S-adenosylmethionine production", RSC ADVANCES, vol. 5, no. 51, 1 January 2015 (2015-01-01), pages 41103 - 41111, XP093070983, DOI: 10.1039/C5RA02937F
CHEN YAWEI: "Enhancing S-Adenosylmethionine Biosynthesis in Saccharomyces Cerevisiae Using a Cofactor Engineering Strategy", CHINESE JOURNAL OF BIOTECHNOLOGY, ZHONGGUO KEXUEYUAN WEISHENGWU YANJIUSUO, CHINESE ACADEMY OF SCIENCES, INSTITUTE OF MICROBIOLOGY, CN, vol. 34, no. 2, 25 February 2018 (2018-02-25), CN , pages 246 - 254, XP093071122, ISSN: 1000-3061, DOI: 10.13345/j.cjb.170174
CHEN YAWEI, XU DUANBIN, FAN LIHAI, ZHANG XU, TAN TIANWEI: "Manipulating multi-system of NADPH regulation in Escherichia coli for enhanced S-adenosylmethionine production", RSC ADVANCES, vol. 5, no. 51, 1 January 2015 (2015-01-01), pages 41103 - 41111, XP093070983, DOI: 10.1039/C5RA02937F
CHEN YAWEI: "Enhancing S-Adenosylmethionine Biosynthesis in Saccharomyces Cerevisiae Using a Cofactor Engineering Strategy", CHINESE JOURNAL OF BIOTECHNOLOGY, ZHONGGUO KEXUEYUAN WEISHENGWU YANJIUSUO, CHINESE ACADEMY OF SCIENCES, INSTITUTE OF MICROBIOLOGY, CN, vol. 34, no. 2, 25 February 2018 (2018-02-25), CN , pages 246 - 254, XP093071122, ISSN: 1000-3061, DOI: 10.13345/j.cjb.170174
Attorney, Agent or Firm:
PERIODIC LAW FIRM (CN)
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