Title:
METHOD IN THE FORM OF A DRY RAPID TEST FOR DETECTING NUCLEIC ACIDS
Document Type and Number:
WIPO Patent Application WO2003039703
Kind Code:
A3
Abstract:
The invention relates to the field of diagnostics of nucleic acids, especially to a highly sensitive method for detecting, differentiating and characterizing nucleic acids in the form of a dry rapid test. The inventive dry rapid test contains a chromatographic material that comprises a sample reception zone, a separation zone including a binding area, in which one or more sequence-specific nucleic acid probes are immobilized, and a zone which absorbs a liquid down-stream of the separation zone including a binding area. According to the inventive method, the sequence-specific nucleic acid probes are immobilized via a polymer linker. The method comprises the following steps: i) denaturing, in the case of double-stranded nucleic acids, the nucleic acid to be detected and then neutralizing it, ii) applying the nucleic acid to be detected to the sample reception zone in a run buffer which contains mildly denaturing agents, iii) the nucleic acid moving from the sample reception zone in direction of the liquid-absorbing zone, (iv) contacting the nucleic acid to be detected in the binding area of the separation path with the sequence-specific nucleic acid probe and hybridizing it with the sequence-specific nucleic acid probe, v) detecting the nucleic acid or the hybridization of the nucleic acid with the sequence-specific nucleic acid probe via a label that is attached to the nucleic acid to be detected or via the detection of a label of the nucleic acid double strand. The invention further relates to a device for carrying out the method according to the invention.
Inventors:
WEIZENEGGER MICHAEL (DE)
Application Number:
PCT/EP2002/012333
Publication Date:
April 01, 2004
Filing Date:
November 05, 2002
Export Citation:
Assignee:
HAIN LIFESCIENCE GMBH (DE)
WEIZENEGGER MICHAEL (DE)
WEIZENEGGER MICHAEL (DE)
International Classes:
G01N33/53; B01D15/00; B01J20/281; B01J20/32; C12M1/00; C12M1/34; C12N15/09; C12Q1/04; C12Q1/68; C12Q1/6834; G01N33/543; G01N33/566; G01N33/569; G01N33/58; G01N30/90; (IPC1-7): B01D15/00; C12Q1/68
Domestic Patent References:
| WO1989011548A1 | 1989-11-30 | |||
| WO1990001564A1 | 1990-02-22 | |||
| WO2002004671A2 | 2002-01-17 | |||
| WO2003040388A2 | 2003-05-15 |
Foreign References:
| US6037127A | 2000-03-14 | |||
| US5212059A | 1993-05-18 | |||
| EP0745689A2 | 1996-12-04 | |||
| US5310650A | 1994-05-10 |
Other References:
WEIZENEGGER M ET AL: "IDENTIFICATION OF MICROORGANISMS ASSOCIATED WITH PERIODONTAL DISEASE BY REVERSE HYBRIDIZATION", JOURNAL OF MICROBIOLOGICAL METHODS, ELSEVIER, AMSTERDAM,, NL, vol. 38, no. 3, 13 October 1999 (1999-10-13), pages 261, XP008020287, ISSN: 0167-7012
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