Title:
METHOD FOR IN VITRO EXPANSION OF ERYTHROID CELLS
Document Type and Number:
WIPO Patent Application WO/2013/147425
Kind Code:
A1
Abstract:
The present invention relates to a method for in vitro expansion of mature erythroid cells. More specifically, the present invention relates to a method for obtaining concentrated erythrocytes by culturing erythroid cells at high density so as to allow the cells to physically and directly come in contact with each other. Particularly, the method of the present invention is very useful in that it is possible to obtain a large amount of clinically useful concentrated erythrocytes through a small container such as a test tube-sized bioreactor.
Inventors:
BAEK EUN-JUNG (KR)
CHOI HYE-SOOK (KR)
CHOI HYE-SOOK (KR)
Application Number:
PCT/KR2013/001650
Publication Date:
October 03, 2013
Filing Date:
February 28, 2013
Export Citation:
Assignee:
IUCF HYU (KR)
BAEK EUN-JUNG (KR)
CHOI HYE-SOOK (KR)
BAEK EUN-JUNG (KR)
CHOI HYE-SOOK (KR)
International Classes:
C12N5/078; C12N5/02; C12N15/85
Other References:
LEE, G. ET AL.: "Targeted gene deletion demonstrates that the cell adhesion molecule ICAM-4 is critical for erythroblastic island formation", BLOOD, vol. 108, no. 6, 15 September 2006 (2006-09-15), pages 2064 - 2071
ZENNADI, R. ET AL.: "Epinephrine acts through erythroid signaling pathways to activate sickle cell adhesion to endothelium via LW-avy3 interactions", BLOOD, vol. 104, no. 12, 1 December 2004 (2004-12-01), pages 3774 - 3781
KUMAR, A. ET AL.: "Phorbol Ester Stimulation Increases Sickle Erythrocyte Adherence to Endothelium: A Novel Pathway Involving a4pI Integrin Receptors on Sickle Reticulocytes and Fibronectin", BLOOD, vol. 88, no. 11, 1 December 1996 (1996-12-01), pages 4348 - 4358
CHOI, HS. ET AL.: "Autonomous control of terminal erythropoiesis via physical interactions among erythroid cells", STEM CELL RESEARCH, vol. 10, 13 February 2013 (2013-02-13), pages 442 - 453
ZENNADI, R. ET AL.: "Epinephrine acts through erythroid signaling pathways to activate sickle cell adhesion to endothelium via LW-avy3 interactions", BLOOD, vol. 104, no. 12, 1 December 2004 (2004-12-01), pages 3774 - 3781
KUMAR, A. ET AL.: "Phorbol Ester Stimulation Increases Sickle Erythrocyte Adherence to Endothelium: A Novel Pathway Involving a4pI Integrin Receptors on Sickle Reticulocytes and Fibronectin", BLOOD, vol. 88, no. 11, 1 December 1996 (1996-12-01), pages 4348 - 4358
CHOI, HS. ET AL.: "Autonomous control of terminal erythropoiesis via physical interactions among erythroid cells", STEM CELL RESEARCH, vol. 10, 13 February 2013 (2013-02-13), pages 442 - 453
Attorney, Agent or Firm:
NAHM, Ho-Hyun (KR)
남호현 (KR)
남호현 (KR)
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