Title:
METHOD FOR IN-VITRO SIMULATION AND EVALUATION OF PLATELET ADHESION IN BLOOD CONTACT MEDICAL INSTRUMENT
Document Type and Number:
WIPO Patent Application WO/2021/077732
Kind Code:
A1
Abstract:
A method for in-vitro simulation and evaluation of platelet adhesion in a blood contact medical instrument, comprising the following steps: (1) simulating viscosity and hydrodynamic characteristics of blood using a glycerin aqueous solution having a concentration of 40% by mass in an extracorporeal circulation loop, and adding fluorescent particles having a diameter of 3-5 microns into the solution to simulate platelets; (2) after the solution circulates in the loop for a certain period of time, disassembling a tested artificial organ or a flow-through part of a medical instrument, and observing a fluorescent particle deposition status on the blood contact surface in the instrument by naked eyes and photography; and (3) using laser-induced fluorescence technology to apply laser on the instrument surface where the fluorescent particles are deposited and which contact the blood, and using a charge-coupled device camera imaging method to photograph an aggregation and adhesion status of laser-induced fluorescent particles. Therefore, a position where platelet adhesion and thrombi are prone to form in an instrument can be located, structural defects of the instrument are analyzed, and improvement and optimization are made in time.
Inventors:
HU SHENGSHOU (CN)
LIU GUANGMAO (CN)
LIU GUANGMAO (CN)
Application Number:
PCT/CN2020/091505
Publication Date:
April 29, 2021
Filing Date:
May 21, 2020
Export Citation:
Assignee:
FUWAI HOSPITAL CHINESE ACAD OF MEDICAL SCIENCES SHENZHEN (CN)
International Classes:
A61F2/24; A61F2/82; G01N21/64
Domestic Patent References:
| WO2000066730A2 | 2000-11-09 |
Foreign References:
| CN101361988A | 2009-02-11 | |||
| CN109900885A | 2019-06-18 | |||
| CN101251434A | 2008-08-27 | |||
| CN101347360A | 2009-01-21 |
Other References:
LIU GUANG-MAO, HAI-BO CHEN, JIAN-FENG HOU, YAN ZHANG AND SHENG-SHOU HU: "Platelet adhesion emulation: A novel method for estimating the device thrombosis potential of a ventricular assist device", THE INTERNATIONAL JOURNAL OF ARTIFICIAL ORGANS IJAO THE INTERNATIONAL JOURNAL OF ARTIFICIAL ORGANS, vol. 43, no. 4, 1 January 2020 (2020-01-01), pages 252 - 257, XP055803775, DOI: 10.1177/0391398819885946
See also references of EP 4062872A4
See also references of EP 4062872A4
Attorney, Agent or Firm:
BEIJING BEIXIN-ZHICHENG INTELLECTUAL PROPERTY AGENT CO., LTD. (CN)
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