Title:
METHOD FOR THE INACTIVATION OF AMYLASE IN THE PRESENCE OF PROTEASE
Document Type and Number:
WIPO Patent Application WO2003052092
Kind Code:
A3
Abstract:
The invention relates to a method for the inactivation of amylase in a solution comprising Rhizomucor miehei aspartic protease by keeping the solution at a pH between 2.1 and 2.8 and at a temperature between 20 DEG C and 40 DEG C for a period of time sufficient to inactivate amylase for at least 95 %.With this method the level of residual Rhizomucor miehei aspartic protease activity in the solution at the end of the heating time is at least 90 %, preferably at least 95 %, and the residual amylase activity is 0.05 RAU/g or lower, more preferably 0.001 RAU/g or lower, i.e. more than 99% of the amylase activity has been inactivated.
Inventors:
CAUSSETTE MYLENE (FR)
MIGGELBRINK FREDERIK (NL)
MAUFROID GREGORY (FR)
MIGGELBRINK FREDERIK (NL)
MAUFROID GREGORY (FR)
Application Number:
PCT/EP2002/014529
Publication Date:
November 06, 2003
Filing Date:
December 18, 2002
Export Citation:
Assignee:
DSM IP ASSETS BV (NL)
CAUSSETTE MYLENE (FR)
MIGGELBRINK FREDERIK (NL)
MAUFROID GREGORY (FR)
CAUSSETTE MYLENE (FR)
MIGGELBRINK FREDERIK (NL)
MAUFROID GREGORY (FR)
International Classes:
A23C19/032; C12N9/30; C12N9/58; C12N9/99; (IPC1-7): C12N9/58; C12N9/30; C12N9/99; A23C19/032
Domestic Patent References:
| WO1997020921A1 | 1997-06-12 |
Foreign References:
| US5800849A | 1998-09-01 | |||
| US3988207A | 1976-10-26 | |||
| US2683682A | 1954-07-13 | |||
| US4591565A | 1986-05-27 | |||
| US4943530A | 1990-07-24 | |||
| US4086139A | 1978-04-25 |
Other References:
STERNBERG M Z: "CRYSTALLINE MILK-CLOTTING PROTEASE FROM MUCOR MIEHEI AND SOME OF ITS PROPERTIES", JOURNAL OF DAIRY SCIENCE, AMERICAN DAIRY SCIENCE ASSOCIATION. CHAMPAIGN, ILLINOIS, US, vol. 2, no. 54, 1971, pages 159 - 167, XP001073721, ISSN: 0022-0302
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