Title:
METHOD OF INCREASING BIOMASS AND LIPID CONTENT IN A MICRO-ORGANISM AND A GENETICALLY MODIFIED MICRO-ORGANISM EXHIBITING ENHANCED AUTOPHAGY
Document Type and Number:
WIPO Patent Application WO/2015/111075
Kind Code:
A3
Abstract:
According to an embodiment of the invention, there is provided a method of increasing biomass and lipid content in a micro-organism comprising cloning in a vector an exogenous gene sequence selected from the group comprising Atg1 gene, Atg6 gene, and Atg8 gene sequence wherein the sequence is codon optimized for said micro-organism, for inducing autophagy; introducing the vector containing the gene into the genome of the micro-organism to yield a genetically modified micro-organism; and growing the genetically modified micro-organism in suitable medium. According to another embodiment of the invention there is provided a method of increasing biomass and lipid content in a micro-organism exposed to stress, comprising treating the microorganism with an autophagy inducing agent.
Inventors:
DAS GAUTAM (IN)
DASGUPTA SANTANU (IN)
KUMAR RAJA (IN)
PRASAD VENKATESH (IN)
DEEPAK NANJAPPA (IN)
SONI NIRAJA (IN)
DATE AMOL (IN)
SONI BADRISH RANJITLAL (IN)
NAGARJUNA PASUPULETI (IN)
DASGUPTA SANTANU (IN)
KUMAR RAJA (IN)
PRASAD VENKATESH (IN)
DEEPAK NANJAPPA (IN)
SONI NIRAJA (IN)
DATE AMOL (IN)
SONI BADRISH RANJITLAL (IN)
NAGARJUNA PASUPULETI (IN)
Application Number:
PCT/IN2015/000011
Publication Date:
December 23, 2015
Filing Date:
January 09, 2015
Export Citation:
Assignee:
RELIANCE IND LTD (IN)
International Classes:
C12N15/79
Foreign References:
| US20120115193A1 | 2012-05-10 | |||
| US20120288918A1 | 2012-11-15 | |||
| US20100173983A1 | 2010-07-08 |
Other References:
HEITZER ET AL.: "Construction of modular tandem expression vectors for the green alga Chlamydomonas reinhardtii using the Cre/lox-system", BIOTECHNIQUES, vol. 43, no. 3, 1 September 2007 (2007-09-01), pages 324 - 332, XP055104012
CHEN ET AL.: "Targeting protective autophagy exacerbates UV-triggered apoptotic cell death", INT J MOL SCI., vol. 13, no. 1, 20 January 2012 (2012-01-20), pages 1209 - 1224, XP055244881
KAPUY ET AL.: "A cellular stress-directed bistable switch controls the crosstalk between autophagy and apoptosis", MOL BIOSYST., vol. 9, no. 2, 7 December 2012 (2012-12-07), pages 296 - 306, XP055244882, DOI: doi:10.1039/C2MB25261A
CHEN ET AL.: "Targeting protective autophagy exacerbates UV-triggered apoptotic cell death", INT J MOL SCI., vol. 13, no. 1, 20 January 2012 (2012-01-20), pages 1209 - 1224, XP055244881
KAPUY ET AL.: "A cellular stress-directed bistable switch controls the crosstalk between autophagy and apoptosis", MOL BIOSYST., vol. 9, no. 2, 7 December 2012 (2012-12-07), pages 296 - 306, XP055244882, DOI: doi:10.1039/C2MB25261A
Attorney, Agent or Firm:
DESOUZA, Fiona (One Indiabulls Centre 13th Flr,841 Senapati Bapat, Marg,Mumbai 3, Maharashtra, IN)
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