Title:
METHOD FOR INDUCING CEREBRAL CORTEX NEURONS
Document Type and Number:
WIPO Patent Application WO/2016/167372
Kind Code:
A1
Abstract:
Provided is a method for producing cerebral cortex neurons from pluripotent stem cells. Provided is a method for producing cerebral cortex neurons from pluripotent stem cells, including: (i) a step for carrying out suspension culture of pluripotent stem cells in a medium that contains a TGFβ inhibitor, bFGF, a Wnt inhibitor, and a BMP inhibitor; (ii) a step for carrying out suspension culture of the cells obtained in step (i) in a medium that contains a Wnt inhibitor and a BMP inhibitor; and (iii) a step for further culturing the cells obtained in step (ii).
Inventors:
TAKAHASHI JUN (JP)
MOTONO MAKOTO (JP)
IOROI YOSHIHIKO (JP)
MOTONO MAKOTO (JP)
IOROI YOSHIHIKO (JP)
Application Number:
PCT/JP2016/062578
Publication Date:
October 20, 2016
Filing Date:
April 14, 2016
Export Citation:
Assignee:
UNIV KYOTO (JP)
International Classes:
C12N5/0797; A61K35/30; A61P25/00; A61P43/00; C12N5/0735; C12N5/074; C12N5/0793; C12N5/10; A61K35/545
Domestic Patent References:
| WO2010144696A1 | 2010-12-16 | |||
| WO2014176606A1 | 2014-10-30 |
Foreign References:
| JP2015019628A | 2015-02-02 |
Other References:
TETSUHIRO KIKUCHI ET AL.: "Fuyu Baiyoho ni yoru Hito iPS Saibo kara no Shinkei Bunka Yudo", ANNUAL MEETING OF THE JAPAN NEUROSURGICAL SOCIETY ABSTRACT, vol. 68 th, 2009
YU KAMISHIBAHARA ET AL.: "Promotion of mouse embryonic stem cell differentiation by Rho kinase inhibitor Y-27632", NEUROSCIENCE LETTERS, vol. 579, 2014, pages 58 - 63, XP029046798
YU KAMISHIBAHARA ET AL.: "Promotion of mouse embryonic stem cell differentiation by Rho kinase inhibitor Y-27632", NEUROSCIENCE LETTERS, vol. 579, 2014, pages 58 - 63, XP029046798
Attorney, Agent or Firm:
HIRAKI, Yusuke et al. (JP)
Yusuke Hiraki (JP)
Yusuke Hiraki (JP)
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