Title:
METHOD FOR INDUCING DIFFERENTIATION OF ARTIFICIAL PLURIPOTENT STEM CELL INTO HEPATOCYTE
Document Type and Number:
WIPO Patent Application WO/2013/183571
Kind Code:
A1
Abstract:
[Problem] To provide: a novel method for inducing the differentiation of an artificial pluripotent stem cell into a hepatocyte with high efficiency; and a use of the method.
[Solution] The differentiation of an artificial pluripotent stem cell into a hepatocyte can be induced through: a step of differentiating the artificial pluripotent stem cell into an endoderm-like cell; and a step of differentiating the endoderm-like cell produced in the preceding step into a hepatocyte-like cell, in which at least a part of the culturing of the endoderm-like cell is carried out in the presence of a histone deacetylase inhibitor and/or under oxidative stress-applied conditions.
Inventors:
KONDO YUKI (JP)
IWAO TAKAHIRO (JP)
MATSUNAGA TAMIHIDE (JP)
YOSHIHASHI SACHIMI (JP)
MIYATA NAOKI (JP)
SUZUKI TAKAYOSHI (JP)
IWAO TAKAHIRO (JP)
MATSUNAGA TAMIHIDE (JP)
YOSHIHASHI SACHIMI (JP)
MIYATA NAOKI (JP)
SUZUKI TAKAYOSHI (JP)
Application Number:
PCT/JP2013/065298
Publication Date:
December 12, 2013
Filing Date:
June 01, 2013
Export Citation:
Assignee:
PUBLIC UNIV CORP NAGOYA CITY UNIV (JP)
International Classes:
C12N5/10; A61K35/12; A61L27/00; A61P1/16; C12N5/071; C12Q1/02; G01N33/15
Domestic Patent References:
| WO2011116930A1 | 2011-09-29 |
Foreign References:
| JP2009100702A | 2009-05-14 |
Other References:
GOTTLICHER,M. ET AL.: "Valproic acid defines a novel class of HDAC inhibitors inducing differentiation of transformed cells.", EMBO J., vol. 20, no. 24, 17 December 2001 (2001-12-17), pages 6969 - 78
PHIEL,C.J. ET AL.: "Histone deacetylase is a direct target of valproic acid, a potent anticonvulsant, mood stabilizer, and teratogen.", J. BIOL. CHEM., vol. 276, no. 39, 28 September 2001 (2001-09-28), pages 36734 - 41
DONG,X.J. ET AL.: "Direct hepatic differentiation of mouse embryonic stem cells induced by valproic acid and cytokines.", WORLD J. GASTROENTEROL., vol. 15, no. 41, 7 November 2009 (2009-11-07), pages 5165 - 75
PHIEL,C.J. ET AL.: "Histone deacetylase is a direct target of valproic acid, a potent anticonvulsant, mood stabilizer, and teratogen.", J. BIOL. CHEM., vol. 276, no. 39, 28 September 2001 (2001-09-28), pages 36734 - 41
DONG,X.J. ET AL.: "Direct hepatic differentiation of mouse embryonic stem cells induced by valproic acid and cytokines.", WORLD J. GASTROENTEROL., vol. 15, no. 41, 7 November 2009 (2009-11-07), pages 5165 - 75
Attorney, Agent or Firm:
HAGINO Mikiharu (JP)
Kanji Hagino (JP)
Kanji Hagino (JP)
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