Title:
METHOD FOR INDUCING DIFFERENTIATION OF CORNEAL EPITHELIAL CELLS FROM PLURIPOTENT STEM CELLS
Document Type and Number:
WIPO Patent Application WO/2016/114285
Kind Code:
A1
Abstract:
The present invention relates to a method for inducing differentiation of corneal epithelial cells from pluripotent stem cells. More specifically, the present invention relates to a method in which pluripotent stem cells such as human iPS cells are made to autonomously differentiate into an ectodermal cell type on a serum-free medium without the use of feeder cells and the differentiation of obtained ocular surface ectodermal lineage cells into corneal epithelial cells is induced.
Inventors:
NISHIDA KOHJI (JP)
HAYASHI RYUHEI (JP)
ISHIKAWA YUKI (JP)
HAYASHI RYUHEI (JP)
ISHIKAWA YUKI (JP)
Application Number:
PCT/JP2016/050784
Publication Date:
July 21, 2016
Filing Date:
January 13, 2016
Export Citation:
Assignee:
UNIV OSAKA (JP)
International Classes:
C12N5/071; C12N5/079; C12N5/10
Domestic Patent References:
| WO2012144582A1 | 2012-10-26 | |||
| WO2012073238A1 | 2012-06-07 |
Other References:
R.HAYASHI ET AL.: "Generation of Corneal Epithelial Cells from Induced Pluripotent Stem Cells Derived from Human Dermal Fibroblast and Corneal Limbal Epithelium", PLOS ONE, vol. 7, no. Issue 9, e45435, 2012, pages 1 - 10, XP055143151
H.SUGA ET AL.: "Self-formation of functional adenohypophysis in three-dimensional culture", NATURE, vol. 480, 2011, pages 57 - 62, XP055067739
T.D.BLALOCK ET AL.: "Functions of MUC16 in Corneal Epithelial Cells", INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, vol. 48, no. 10, 2007, pages 4509 - 4518, XP055464527
R.HAYASHI ET AL.: "Co-ordinated ocular development from human iPS cells and recovery of corneal function", NATURE, vol. 531, March 2016 (2016-03-01), pages 376 - 380, XP055383265
See also references of EP 3246394A4
H.SUGA ET AL.: "Self-formation of functional adenohypophysis in three-dimensional culture", NATURE, vol. 480, 2011, pages 57 - 62, XP055067739
T.D.BLALOCK ET AL.: "Functions of MUC16 in Corneal Epithelial Cells", INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, vol. 48, no. 10, 2007, pages 4509 - 4518, XP055464527
R.HAYASHI ET AL.: "Co-ordinated ocular development from human iPS cells and recovery of corneal function", NATURE, vol. 531, March 2016 (2016-03-01), pages 376 - 380, XP055383265
See also references of EP 3246394A4
Attorney, Agent or Firm:
OHNO, Seiji et al. (JP)
Seiji Ono (JP)
Seiji Ono (JP)
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