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Title:
METHOD FOR INDUCING DIFFERENTIATION INTO AND PRODUCING BEIGE AND WHITE ADIPOCYTES
Document Type and Number:
WIPO Patent Application WO/2019/098310
Kind Code:
A1
Abstract:
The present invention provides a unilocular adipocyte manufacturing method that includes suspension cultivating mesenchymal cells that have the ability to differentiate into an adipocyte, and inducing differentiation into unilocular adipocytes, said suspension cultivation being in a liquid culture medium composition that allows suspension cultivation of cells or tissue and includes a polymer compound having an anionic functional group and forming a structure that can suspend the cells or tissue by bonding thereto via a divalent metal cation, wherein the polymer compound is a polysaccharide, preferably a polysaccharide including a glucuronic acid site, and more preferably deacylated gellan gum, diutan gum, xanthan gum, or a salt thereof.

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Inventors:
KANAKI TATSURO (JP)
Application Number:
PCT/JP2018/042388
Publication Date:
May 23, 2019
Filing Date:
November 16, 2018
Export Citation:
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Assignee:
NISSAN CHEMICAL CORP (JP)
International Classes:
C12N5/077; C12N5/0775
Domestic Patent References:
WO2007004469A12007-01-11
WO2014017513A12014-01-30
WO2014017513A12014-01-30
WO2016121896A12016-08-04
WO2016125884A12016-08-11
WO2017057599A12017-04-06
WO2017154952A12017-09-14
WO2014017513A12014-01-30
WO2017154952A12017-09-14
Foreign References:
US20140106348A12014-04-17
US20140106348A12014-04-17
US20160060601A12016-03-03
US20140106348A12014-04-17
JP2017221360A2017-12-21
Other References:
WANG, Y. H. ET AL.: "Characterization and evaluation of the differentiation ability of human adipose-derived stem cells srowing in scaffold- free suspension culture", CYTOTHERAPY, vol. 16, no. 4, April 2014 (2014-04-01), pages 485 - 495, XP055609344
INT J OBES RELAT METAB DISORD., vol. 20, no. 3, March 1996 (1996-03-01), pages 77 - 83
DIABETES, vol. 45, 1996, pages 1435 - 1438
See also references of EP 3708654A4
Attorney, Agent or Firm:
TAKASHIMA, Hajime et al. (JP)
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