Title:
METHOD FOR INDUCING DIFFERENTIATION OF IPSCS TO OBTAIN CD34+ CELLS AND NK CELLS AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2023/240763
Kind Code:
A1
Abstract:
Disclosed in the present invention are a method for inducing the differentiation of iPSCs to obtain CD34+ cells and NK cells and the use thereof. According to the method, using the three-dimensional structure of an embryoid body to provide a good differentiation microenvironment for the differentiation of iPSC, a high proportion of CD34+ cells can be produced on day 4 under hypoxia-induced culture conditions, and subsequently, by means of inducing the differentiation via embryoid body adhesion or digestion and resuspension, the yield of NK cells obtained by the induced differentiation of iPSCs is significantly improved, and the NK cells obtained by means of the induced differentiation can play a role in killing tumor cells in a short time, and have strong tumor-killing ability. The method is suitable for the large-scale production and clinical application of cell preparations.
Inventors:
GONG SHIXIN (CN)
GU YUCHUN (CN)
LI NAN (CN)
JIANG MINGYUE (CN)
CAO WENHUA (CN)
PENG QINQING (CN)
WU LIDA (CN)
GU YUCHUN (CN)
LI NAN (CN)
JIANG MINGYUE (CN)
CAO WENHUA (CN)
PENG QINQING (CN)
WU LIDA (CN)
Application Number:
PCT/CN2022/109959
Publication Date:
December 21, 2023
Filing Date:
August 03, 2022
Export Citation:
Assignee:
ALLIFE MEDICINE BEIJING LTD (CN)
International Classes:
A61K35/17; C12N5/10; A61P7/00; A61P35/00; A61P37/02; C12N5/0783; C12N5/0789
Foreign References:
| CN114774365A | 2022-07-22 | |||
| CN112608895A | 2021-04-06 | |||
| US20200080059A1 | 2020-03-12 | |||
| CN112226409A | 2021-01-15 | |||
| CN107429230A | 2017-12-01 | |||
| CN107338220A | 2017-11-10 |
Other References:
SUDO KAZUHIRO; YAMAZAKI SATOSHI; WILKINSON ADAM C.; NAKAUCHI HIROMITSU; NAKAMURA YUKIO: "Polyvinyl alcohol hydrolysis rate and molecular weight influence human and murine HSC activity ex vivo", STEM CELL RESEARCH, ELSEVIER, vol. 56, no. 56, 3 September 2021 (2021-09-03), NL , XP086842141, ISSN: 1873-5061, DOI: 10.1016/j.scr.2021.102531
JIAN WANG, LIN GE, ZHAO HUI-PING, LU GUANG-XIU : "Spontaneous differentiation of human embryonic stem cells into hematopoietic cells", JOURNAL OF SOUTHERN MEDICAL UNIVERSITY, vol. 29, no. 4, 20 April 2009 (2009-04-20), pages 602 - 605, 610, XP093118623
JIAN WANG, LIN GE, ZHAO HUI-PING, LU GUANG-XIU : "Spontaneous differentiation of human embryonic stem cells into hematopoietic cells", JOURNAL OF SOUTHERN MEDICAL UNIVERSITY, vol. 29, no. 4, 20 April 2009 (2009-04-20), pages 602 - 605, 610, XP093118623
Attorney, Agent or Firm:
BEIJING ZHI CHENG HAN FANG IP AGENCY CO., LTD. (CN)
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