Title:
METHOD OF INDUCING DIFFERENTIATION OF MESOBLAST STEM CELLS OR ES CELLS INTO NERVE SYSTEM CELLS
Document Type and Number:
WIPO Patent Application WO/2003/038074
Kind Code:
A1
Abstract:
It is found out that when mesoblast stem cells or ES cells prepared from a mononuclear fraction separated from bone marrow fluid or cord blood are cultured in a fundamental liquid culture medium, the differentiation of these mesoblast stem cells or ES cells into nerve system cells or glial cells is induced. It is further found out that the induction of the differentiation of mesoblast stem cells or ES cells into nerve system cells is promoted by adding an ischemic brain extract to the above−described fundamental liquid culture medium. It is clarified that the nerve system cells obtained by the above method are capable of regenerating nerve in a cerebral infarction model, a dementia model, a spinal injury model and a demyelination model.
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Inventors:
HONMOU OSAMU (JP)
Application Number:
PCT/JP2001/009510
Publication Date:
May 08, 2003
Filing Date:
October 30, 2001
Export Citation:
Assignee:
HONMOU OSAMU (JP)
RENOMEDIX INST INC (JP)
RENOMEDIX INST INC (JP)
International Classes:
A61K35/12; A61K35/14; A61K35/28; A61K35/34; A61K35/36; A61K35/51; A61K35/545; A61K35/76; A61P25/00; C12N5/074; C12N5/079; (IPC1-7): C12N5/06; A61K35/28; A61P25/00
Other References:
SASAKI M. ET AL.: "Transplantation of an acutely isolated bone marrow fraction repairs demyelinated adult rat spinal cord axons", GLIA, vol. 35, no. 1, 2001, pages 26 - 34, XP002945994
TREMAIN N. ET AL.: "MicroSAGE analysis of 2,353 expressed genes in a single cell-derived colony of undifferentiated human mesenchymal stem cells reveals mRNAs of multiple cell lineages", STEM CELLS, vol. 19, 2001, pages 408 - 418, XP002907879
BRUESTLE O. ET AL.: "Embryonic stem cell-derived glial precursors: a source of myelinating transplants", SCIENCE, vol. 285, 1999, pages 754 - 756, XP002907880
PITTENGER M.F. ET AL.: "Multilineage potential of adult human mesenchymal stem cells", SCIENCE, vol. 284, 1999, pages 143 - 147, XP000867221
TREMAIN N. ET AL.: "MicroSAGE analysis of 2,353 expressed genes in a single cell-derived colony of undifferentiated human mesenchymal stem cells reveals mRNAs of multiple cell lineages", STEM CELLS, vol. 19, 2001, pages 408 - 418, XP002907879
BRUESTLE O. ET AL.: "Embryonic stem cell-derived glial precursors: a source of myelinating transplants", SCIENCE, vol. 285, 1999, pages 754 - 756, XP002907880
PITTENGER M.F. ET AL.: "Multilineage potential of adult human mesenchymal stem cells", SCIENCE, vol. 284, 1999, pages 143 - 147, XP000867221
Attorney, Agent or Firm:
Shimizu, Hatsushi (1-1-1 Oroshi-mach, Tsuchiura-shi Ibaraki, JP)
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