Title:
METHOD FOR INDUCING DIFFERENTIATION OF PLURIPOTENT STEM CELLS INTO GERM CELLS
Document Type and Number:
WIPO Patent Application WO/2017/002888
Kind Code:
A1
Abstract:
The purpose of the present invention is to provide a method for inducing the differentiation of human pluripotent stem cells into human primordial germ cell-like (PGC-like) cells at a high efficiency and high reproductivity, and a cell surface marker for specifying human PGC-like cells.
To achieve the above purpose, provided is a method for producing human PGC-like cells from human pluripotent stem cells, said method comprising: (I) a step for culturing human pluripotent stem cells in a liquid culture medium containing activin A and a GSK3β inhibitor to give mesoderm-like cells; and (II) a step for culturing the mesoderm-like cells obtained in step (I) in a liquid culture medium containing BMP. Also provided is a method for producing isolated human PGC-like cells, said method comprising: (III) a step for, among the cells obtained in the aforesaid step (II), screening cells positive for at least one cell surface maker selected from the group consisting of PECAM (CD31), INTEGRINα6 (CD49f), INTEGRINβ3 (CD61), KIT (CD117), EpCAM, PODOPLANIN and TRA1-81.
Inventors:
SAITOU MITINORI (JP)
SASAKI KOTARO (JP)
YOKOBAYASHI SHIHORI (JP)
SASAKI KOTARO (JP)
YOKOBAYASHI SHIHORI (JP)
Application Number:
PCT/JP2016/069360
Publication Date:
January 05, 2017
Filing Date:
June 29, 2016
Export Citation:
Assignee:
UNIV KYOTO (JP)
International Classes:
C12N5/071; A61K35/545
Domestic Patent References:
| WO2014174470A1 | 2014-10-30 |
Foreign References:
| JP2013530680A | 2013-08-01 | |||
| JP2013538038A | 2013-10-10 |
Other References:
IRIE NAOKO ET AL.: "SOX17 Is a Critical Specifier of Human Primordial Germ Cell Fate", CELL, vol. 160, no. 1, 15 January 2015 (2015-01-15), pages 253 - 268, XP029132652
SUGAWA FUMIHIRO ET AL.: "Human primordial germ cell commitment in vitro associates with a unique PRDM14 expression profile", THE EMBO JOURNAL, vol. 34, no. 8, 6 March 2015 (2015-03-06), pages 1009 - 1024
PARK S ET AL.: "Preliminary evaluation of PGC differentiation protocol in rhesus model.", FERTILITY AND STERILITY, vol. 102, no. 3, September 2014 (2014-09-01), pages e103, XP055340693
SUGAWA FUMIHIRO ET AL.: "Human primordial germ cell commitment in vitro associates with a unique PRDM14 expression profile", THE EMBO JOURNAL, vol. 34, no. 8, 6 March 2015 (2015-03-06), pages 1009 - 1024
PARK S ET AL.: "Preliminary evaluation of PGC differentiation protocol in rhesus model.", FERTILITY AND STERILITY, vol. 102, no. 3, September 2014 (2014-09-01), pages e103, XP055340693
Attorney, Agent or Firm:
TAKASHIMA, Hajime (JP)
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