Title:
METHOD FOR INDUCING GLIAL CELLS TRANSDIFFERENTIATION INTO FUNCTIONAL NEURONS, AND APPLICATION THEREOF
Document Type and Number:
WIPO Patent Application WO/2021/228050
Kind Code:
A1
Abstract:
Provided is a use of a Neurog2 functional fragment. The functional fragment can induce, in vivo or in vitro, glial cell formation into functional neuron cells, and thus can not only have a transdifferentiation function in normal tissue, but also facilitate neural reconstruction of damaged neural tissue.
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Inventors:
CHEN RULEI (CN)
LIU TING (CN)
LIU TING (CN)
Application Number:
PCT/CN2021/092851
Publication Date:
November 18, 2021
Filing Date:
May 10, 2021
Export Citation:
Assignee:
NEURAGEN BIOTHERAPEUTICS CO LTD (CN)
International Classes:
C12N5/0793; A61P25/00; C12N15/63
Other References:
CHOUCHANE MALEK, MELO DE FARIAS ANA RAQUEL, MOURA DANIELA MARIA DE SOUSA, HILSCHER MARKUS MICHAEL, SCHROEDER TIMM, LEÃO RICHARDSON: "Lineage Reprogramming of Astroglial Cells from Different Origins into Distinct Neuronal Subtypes", STEM CELL REPORTS, vol. 9, no. 1, 11 July 2017 (2017-07-11), United States, pages 162 - 176, XP055866413, ISSN: 2213-6711, DOI: 10.1016/j.stemcr.2017.05.009
HEINRICH CHRISTOPHE, GASCÓN SERGIO, MASSERDOTTI GIACOMO, LEPIER ALEXANDRA, SANCHEZ RODRIGO, SIMON-EBERT TATIANA, SCHROEDER TIMM, G: "Generation of subtype-specific neurons from postnatal astroglia of the mouse cerebral cortex", NATURE PROTOCOLS, vol. 6, no. 2, 1 February 2011 (2011-02-01), GB, pages 214 - 228, XP055866417, ISSN: 1750-2799, DOI: 10.1038/nprot.2010.188
CHRISTOPHE HEINRICH, ROBERT BLUM , SERGIO GASCON, GIACOMO MASSERDOTTI1., PRATIBHA TRIPATHI , RODRIGO SANCHEZ , STEFFEN TIEDT , TIM: "Directing astroglia from the cerebral cortex into subtype specific functional neurons", PLOS BIOLOGY, vol. 8, no. 5, 18 May 2010 (2010-05-18), pages 1 - 29, XP055866420, ISSN: 1545-7885, DOI: 10.1371/journal.pbio.1000373
BERNINGER B., COSTA M. R., KOCH U., SCHROEDER T., SUTOR B., GROTHE B., GOTZ M: "Functional properties of neurons derived from in vitro reprogrammed postnatal astroglia", JOURNAL OF NEUROSCIENCE, vol. 27, no. 32, 8 August 2007 (2007-08-08), US, pages 8654 - 8664, XP055866422, ISSN: 1529-2401, DOI: 10.1523/JNEUROSCI.1615-07.2007
QIAN TENG-DA; ZHANG BIN; LIU YAN: "Research on Preparation and Neuronal Induction of Reprogrammed Astrocytes in Vitro", JOURNAL OF CLINICAL NEUROSURGERY, vol. 12, no. 4, 11 August 2015 (2015-08-11), CN, pages 256 - 260+264, XP009531765, ISSN: 1672-7770
XU XIN-LONG; XIE QING-SONG; PAN HONG-SONG; WEI XIAO-JIE; CHEN ZAI-FENG: "Neurogenin2 Gene-Regulated Schwann Cells Differentiate Into Neurons", CHINESE JOURNAL OF TISSUE ENGINEERING RESEARCH , vol. 17, no. 49, 3 December 2013 (2013-12-03), pages 8590 - 8595, XP009531773, ISSN: 2095-4344, DOI: 10.3969/j.issn.2095-4344.2013.49.020
DATABASE Protein 13 November 2001 (2001-11-13), ANONYMOUS: "neurogenin 2 [Mus musculus]", XP055866428, retrieved from Genbank Database accession no. AAG40769
"NCBI", Database accession no. NM_ 024019.4
VIERBUCHEN, T ET AL.: "Direct conversion of fibroblasts to functional neurons by defined factors", NATURE, vol. 463, 2010, pages 1035 - 1041, XP055023150, DOI: 10.1038/nature08797
EUROPEAN JOURNAL OF PHARMACOLOGY, vol. 463, no. 1-3, 2003, pages 3 - 33
PROC NATL ACAD SCI U S A, vol. 93, pages 11382 - 11388
TISCOMIA, GSINGER, O.VERMA: "Production and purification of lentiviral vectors", I.M. NAT. PROTOC, vol. 1, 2006, pages 241 - 245
MCDONOUGH AMONTERRUBIO AARIZA J ET AL.: "Calibrated Forceps Model of Spinal Cord Compression Injury", JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, 2015
BASSO, D.M ET AL., J NEUROTRAUMA, 2006
HEINRICH CHRISTOPHE, GASCÓN SERGIO, MASSERDOTTI GIACOMO, LEPIER ALEXANDRA, SANCHEZ RODRIGO, SIMON-EBERT TATIANA, SCHROEDER TIMM, G: "Generation of subtype-specific neurons from postnatal astroglia of the mouse cerebral cortex", NATURE PROTOCOLS, vol. 6, no. 2, 1 February 2011 (2011-02-01), GB, pages 214 - 228, XP055866417, ISSN: 1750-2799, DOI: 10.1038/nprot.2010.188
CHRISTOPHE HEINRICH, ROBERT BLUM , SERGIO GASCON, GIACOMO MASSERDOTTI1., PRATIBHA TRIPATHI , RODRIGO SANCHEZ , STEFFEN TIEDT , TIM: "Directing astroglia from the cerebral cortex into subtype specific functional neurons", PLOS BIOLOGY, vol. 8, no. 5, 18 May 2010 (2010-05-18), pages 1 - 29, XP055866420, ISSN: 1545-7885, DOI: 10.1371/journal.pbio.1000373
BERNINGER B., COSTA M. R., KOCH U., SCHROEDER T., SUTOR B., GROTHE B., GOTZ M: "Functional properties of neurons derived from in vitro reprogrammed postnatal astroglia", JOURNAL OF NEUROSCIENCE, vol. 27, no. 32, 8 August 2007 (2007-08-08), US, pages 8654 - 8664, XP055866422, ISSN: 1529-2401, DOI: 10.1523/JNEUROSCI.1615-07.2007
QIAN TENG-DA; ZHANG BIN; LIU YAN: "Research on Preparation and Neuronal Induction of Reprogrammed Astrocytes in Vitro", JOURNAL OF CLINICAL NEUROSURGERY, vol. 12, no. 4, 11 August 2015 (2015-08-11), CN, pages 256 - 260+264, XP009531765, ISSN: 1672-7770
XU XIN-LONG; XIE QING-SONG; PAN HONG-SONG; WEI XIAO-JIE; CHEN ZAI-FENG: "Neurogenin2 Gene-Regulated Schwann Cells Differentiate Into Neurons", CHINESE JOURNAL OF TISSUE ENGINEERING RESEARCH , vol. 17, no. 49, 3 December 2013 (2013-12-03), pages 8590 - 8595, XP009531773, ISSN: 2095-4344, DOI: 10.3969/j.issn.2095-4344.2013.49.020
DATABASE Protein 13 November 2001 (2001-11-13), ANONYMOUS: "neurogenin 2 [Mus musculus]", XP055866428, retrieved from Genbank Database accession no. AAG40769
"NCBI", Database accession no. NM_ 024019.4
VIERBUCHEN, T ET AL.: "Direct conversion of fibroblasts to functional neurons by defined factors", NATURE, vol. 463, 2010, pages 1035 - 1041, XP055023150, DOI: 10.1038/nature08797
EUROPEAN JOURNAL OF PHARMACOLOGY, vol. 463, no. 1-3, 2003, pages 3 - 33
PROC NATL ACAD SCI U S A, vol. 93, pages 11382 - 11388
TISCOMIA, GSINGER, O.VERMA: "Production and purification of lentiviral vectors", I.M. NAT. PROTOC, vol. 1, 2006, pages 241 - 245
MCDONOUGH AMONTERRUBIO AARIZA J ET AL.: "Calibrated Forceps Model of Spinal Cord Compression Injury", JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, 2015
BASSO, D.M ET AL., J NEUROTRAUMA, 2006
Attorney, Agent or Firm:
XU&PARTNERS, LLC. (CN)
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