Title:
METHOD FOR INDUCING NEURONAL SYNAPSE FORMATION AND MICROBEADS USED IN SAID METHOD
Document Type and Number:
WIPO Patent Application WO/2021/006075
Kind Code:
A1
Abstract:
The purpose of the present invention is to provide a method for inducing a presynapse formation in human neurons. According to the present invention, provided are: a method for inducing a presynapse formation in human neurons, the method comprising co-culturing the peripheral neurons with at least one LRRTM (Leucine-rich repeat transmembrane neuronal protein) molecule selected from the group consisting of LRRTM family molecules or microbeads of which a fusion protein including the same is immobilized on the surface (here, the LRRTM molecules or the fusion protein including the molecules are immobilized via a linker on the surface of the microbeads); and microbeads used in said method.
Inventors:
YUMOTO NORIHIRO (JP)
KAWADA JIRO (JP)
KAWADA JIRO (JP)
Application Number:
PCT/JP2020/025313
Publication Date:
January 14, 2021
Filing Date:
June 26, 2020
Export Citation:
Assignee:
JIKSAK BIOENGINEERING INC (JP)
International Classes:
C07K14/705; C07K16/00; C07K17/06; C07K19/00; C12M1/00; C12M3/00; C12N5/0793
Domestic Patent References:
| WO2003046141A2 | 2003-06-05 | |||
| WO2017187696A1 | 2017-11-02 |
Foreign References:
| US20160137707A1 | 2016-05-19 |
Other References:
DE WIT J. ET AL.: "LRRTM2 interacts with neurexinl and regulates excitatory synapse formation", NEURON, vol. 64, 2009, pages 799 - 806, XP029038387, DOI: 10.1016/j.neuron.2009.12.019
KONDO, TAKUYA ET AL.: "In vitro assessment of drug-induced effects on iPS cell-derived dopaminergic neurons using microelectrode array system", THE 45TH ANNUAL MEETING OF THE JAPANESE SOCIETY OF TOXICOLOGY, vol. 45, 2018, XP055784058, DOI: 10.14869/toxpt.45.1.0_P-193
LINHOFF MICHAEL W., LAURÉN JUHA, CASSIDY ROBERT M., DOBIE FREDERICK A., TAKAHASHI HIDETO, NYGAARD HAAKON B., AIRAKSINEN MATTI S., : "An unbiased expression screen for synaptogenic proteins identifies the LRRTM protein family as synaptic organizers", NEURON, vol. 61, no. 5, 2009, pages 734 - 749, XP055784061, DOI: 10.1016/j.neuron.2009.01.017
YAMAGATA A., ATSUSHI, GOTO-ITO SAKURAKO, SATO YUSUKE, SHIROSHIMA TOMOKO, MAEDA ASAMI, WATANABE MASAHIKO, SAITOH TAKASHI, MAENAKA : "Structural insights into modulation and selectivity of transsynaptic neurexin-LRRTM interaction", NAT. COMMUN., vol. 9, no. 1, 2018, pages 3964, XP055784065, DOI: 10.1038/s41467-018-06333-8
SUDHOF TC: "Towards an Understanding of Synapse Formation", NEURON, vol. 100, no. 2, 24 October 2018 (2018-10-24), pages 276 - 293, XP085512700, DOI: 10.1016/j.neuron.2018.09.040
UEMURA ET AL.: "Trans-synaptic interaction of GluRdelta2 and Neurexin through Cblnl mediates synapse formation in the cerebellum", CELL, vol. 141, no. 6, 11 June 2010 (2010-06-11), pages 1068 - 79
SIDDIQUI ET AL.: "An LRRTM4-HSPG complex mediates excitatory synapse development on dentate gyrus granule cells", NEURON, vol. 79, no. 4, 21 August 2013 (2013-08-21), pages 680 - 95, XP028696478, DOI: 10.1016/j.neuron.2013.06.029
YUMOTOET: "Lrp4 is a retrograde signal for presynaptic differentiation at neuromuscular synapses", NATURE, vol. 489, no. 7416, 20 September 2012 (2012-09-20), pages 438 - 42
LINHOFF ET AL.: "An unbiased expression screen for synaptogenic proteins identifies the LRRTM protein family as synaptic organizers", NEURON, vol. 61, no. 5, 12 March 2009 (2009-03-12), pages 734 - 49, XP055784061, DOI: 10.1016/j.neuron.2009.01.017
J. KOET: "LRRTM2 Function as a Neurexin Ligandd in Promoting Excitatory Synapse Formation", NEURON, vol. 64, 2009, pages 791 - 798
CHAMBERS, STUART M. ET AL.: "Highly efficient neural conversion of human ES and iPS cells by dual inhibition of SMAD signaling", NATURE BIOTECHNOLOGY, vol. 27, no. 3, 2009, pages 275, XP055007827, DOI: 10.1038/nbt.1529
SHI, YICHEN ET AL.: "Human cerebral cortex development from pluripotent stem cells to functional excitatory synapses", NATURE NEUROSCIENCE, vol. 15, no. 3, 2012, pages 477
KONDO, TAKUYA ET AL.: "In vitro assessment of drug-induced effects on iPS cell-derived dopaminergic neurons using microelectrode array system", THE 45TH ANNUAL MEETING OF THE JAPANESE SOCIETY OF TOXICOLOGY, vol. 45, 2018, XP055784058, DOI: 10.14869/toxpt.45.1.0_P-193
LINHOFF MICHAEL W., LAURÉN JUHA, CASSIDY ROBERT M., DOBIE FREDERICK A., TAKAHASHI HIDETO, NYGAARD HAAKON B., AIRAKSINEN MATTI S., : "An unbiased expression screen for synaptogenic proteins identifies the LRRTM protein family as synaptic organizers", NEURON, vol. 61, no. 5, 2009, pages 734 - 749, XP055784061, DOI: 10.1016/j.neuron.2009.01.017
YAMAGATA A., ATSUSHI, GOTO-ITO SAKURAKO, SATO YUSUKE, SHIROSHIMA TOMOKO, MAEDA ASAMI, WATANABE MASAHIKO, SAITOH TAKASHI, MAENAKA : "Structural insights into modulation and selectivity of transsynaptic neurexin-LRRTM interaction", NAT. COMMUN., vol. 9, no. 1, 2018, pages 3964, XP055784065, DOI: 10.1038/s41467-018-06333-8
SUDHOF TC: "Towards an Understanding of Synapse Formation", NEURON, vol. 100, no. 2, 24 October 2018 (2018-10-24), pages 276 - 293, XP085512700, DOI: 10.1016/j.neuron.2018.09.040
UEMURA ET AL.: "Trans-synaptic interaction of GluRdelta2 and Neurexin through Cblnl mediates synapse formation in the cerebellum", CELL, vol. 141, no. 6, 11 June 2010 (2010-06-11), pages 1068 - 79
SIDDIQUI ET AL.: "An LRRTM4-HSPG complex mediates excitatory synapse development on dentate gyrus granule cells", NEURON, vol. 79, no. 4, 21 August 2013 (2013-08-21), pages 680 - 95, XP028696478, DOI: 10.1016/j.neuron.2013.06.029
YUMOTOET: "Lrp4 is a retrograde signal for presynaptic differentiation at neuromuscular synapses", NATURE, vol. 489, no. 7416, 20 September 2012 (2012-09-20), pages 438 - 42
LINHOFF ET AL.: "An unbiased expression screen for synaptogenic proteins identifies the LRRTM protein family as synaptic organizers", NEURON, vol. 61, no. 5, 12 March 2009 (2009-03-12), pages 734 - 49, XP055784061, DOI: 10.1016/j.neuron.2009.01.017
J. KOET: "LRRTM2 Function as a Neurexin Ligandd in Promoting Excitatory Synapse Formation", NEURON, vol. 64, 2009, pages 791 - 798
CHAMBERS, STUART M. ET AL.: "Highly efficient neural conversion of human ES and iPS cells by dual inhibition of SMAD signaling", NATURE BIOTECHNOLOGY, vol. 27, no. 3, 2009, pages 275, XP055007827, DOI: 10.1038/nbt.1529
SHI, YICHEN ET AL.: "Human cerebral cortex development from pluripotent stem cells to functional excitatory synapses", NATURE NEUROSCIENCE, vol. 15, no. 3, 2012, pages 477
Attorney, Agent or Firm:
OSAWA, Kenichi (JP)
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