Title:
METHOD FOR INDUCING PROTEOLYSIS IN EUKARYOTIC CELL
Document Type and Number:
WIPO Patent Application WO/2013/073653
Kind Code:
A1
Abstract:
Provided are: a novel protein-inducible eukaryotic cell which does not kill a host such as budding yeast and has a satisfactory decomposition ability; and a method for inducing proteolysis using the protein-inducible eukaryotic cell.
A proteolysis-inducible non-plant eukaryotic cell, which has a plant-derived TIR1 family protein gene and a chimeric gene capable of expressing a desired protein labeled with a partial sequence of a plant-derived Aux/IAA family protein, and in which the degradation of the desired protein can be induced by an auxin, said non-plant eukaryotic cell being characterized in that the partial sequence is a sequence comprising a region containing at least two Lys residues on each of the N-terminal side and the C-terminal side of a domain II region of an Aux/IAA family protein or a sequence composed of a linked product of two or more of the aforementioned sequences.
Inventors:
KANEMAKI MASATO (JP)
NISHIMURA KOHEI (JP)
TAKISAWA HARUHIKO (JP)
MIMURA SATORU (JP)
KOBATA YUI (JP)
NISHIMURA KOHEI (JP)
TAKISAWA HARUHIKO (JP)
MIMURA SATORU (JP)
KOBATA YUI (JP)
Application Number:
PCT/JP2012/079744
Publication Date:
May 23, 2013
Filing Date:
November 16, 2012
Export Citation:
Assignee:
RES ORG INFORMATION & SYSTEMS (JP)
UNIV OSAKA (JP)
UNIV OSAKA (JP)
International Classes:
C12N15/09; C12N1/15; C12N1/19; C12N5/10
Domestic Patent References:
| WO2010125620A1 | 2010-11-04 |
Foreign References:
| JP2008187958A | 2008-08-21 | |||
| JP2003534775A | 2003-11-25 | |||
| US6222095B1 | 2001-04-24 |
Other References:
DREHER KA ET AL.: "The Arabidopsis Aux/IAA Protein Family Has Diversified in Degradation and Auxin Responsiveness", THE PLANT CELL, vol. 18, 2006, pages 699 - 714
CHU BW ET AL.: "Recent Progress with FKBP- derived destabilizing domains", BIOORG MED CHEM LETT., vol. 18, 2008, pages 5941 - 5944, XP025627175, DOI: doi:10.1016/j.bmcl.2008.09.043
CHU BW ET AL.: "Recent Progress with FKBP- derived destabilizing domains", BIOORG MED CHEM LETT., vol. 18, 2008, pages 5941 - 5944, XP025627175, DOI: doi:10.1016/j.bmcl.2008.09.043
Attorney, Agent or Firm:
THE PATENT CORPORATE BODY ARUGA PATENT OFFICE (JP)
Patent business corporation Alga patent firm (JP)
Patent business corporation Alga patent firm (JP)
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Claims: