Title:
METHOD FOR INDUCING VASCULAR ENDOTHELIAL CELLS
Document Type and Number:
WIPO Patent Application WO/2017/014165
Kind Code:
A1
Abstract:
Provided is a method for producing vascular endothelial cells from pluripotent stem cells, said method comprising: (i) a step for culturing the pluripotent stem cells in a BMP-containing liquid culture medium on a culture tool that is coated with a first matrix to give mesodermal progenitor cells; (ii) a step for dissociating the cells into single cells; and (iii) a step for culturing the cells in a VEGF-containing liquid culture medium on a culture container that is coated with a second matrix selected from the group consisting of laminin-411 or a fragment thereof, laminin-511 or a fragment thereof, Matrigel, IV-type collagen and fibronectin.
Inventors:
NAKAHATA TATSUTOSHI (JP)
SAITO MEGUMU (JP)
NIWA AKIRA (JP)
OTA RYO (JP)
SEKIGUCHI KIYOTOSHI (JP)
SAITO MEGUMU (JP)
NIWA AKIRA (JP)
OTA RYO (JP)
SEKIGUCHI KIYOTOSHI (JP)
Application Number:
PCT/JP2016/070908
Publication Date:
January 26, 2017
Filing Date:
July 14, 2016
Export Citation:
Assignee:
UNIV KYOTO (JP)
UNIV OSAKA (JP)
UNIV OSAKA (JP)
International Classes:
C12N5/10; A61K35/44; A61L27/00; A61P9/10; C07K14/78; C12N5/0735; C12N5/078; C12N15/09
Domestic Patent References:
| WO2002048349A1 | 2002-06-20 | |||
| WO2014103534A1 | 2014-07-03 |
Foreign References:
| US20150017724A1 | 2015-01-15 |
Other References:
DESCAMPS, B. ET AL.: "Vascular differentiation from embryonic stem cells: novel technologies and therapeutic promises", VASCUL.PHARMACOL., vol. 56, no. 5-6, 2012, pages 267 - 279, XP055347085
NIWA, A. ET AL.: "A novel serum-free monolayer culture for orderly hematopoietic differentiation of human pluripotent cells via mesodermal progenitors", PLOS ONE, vol. 6, no. 7, 2011, pages e22261, XP055128572
SCHNAPER H.W. ET AL.: "Role of laminin in endothelial cell recognition and differentiation", KIDNEY INT., vol. 43, no. 1, 1993, pages 20 - 25, XP055347089
MIYAZAKI, T. ET AL.: "Laminin E8 fragments support efficient adhesion and expansion of dissociated human pluripotent stem cells", NAT.COMMUN., vol. 3, 2012, pages 1236
See also references of EP 3327118A4
NIWA, A. ET AL.: "A novel serum-free monolayer culture for orderly hematopoietic differentiation of human pluripotent cells via mesodermal progenitors", PLOS ONE, vol. 6, no. 7, 2011, pages e22261, XP055128572
SCHNAPER H.W. ET AL.: "Role of laminin in endothelial cell recognition and differentiation", KIDNEY INT., vol. 43, no. 1, 1993, pages 20 - 25, XP055347089
MIYAZAKI, T. ET AL.: "Laminin E8 fragments support efficient adhesion and expansion of dissociated human pluripotent stem cells", NAT.COMMUN., vol. 3, 2012, pages 1236
See also references of EP 3327118A4
Attorney, Agent or Firm:
KAWAGUCHI, Yoshiyuki et al. (JP)
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