Title:
METHOD FOR INTRODUCING EXOGENOUS SUBSTANCE INTO CELL, AND MATERIAL USED IN SAID METHOD
Document Type and Number:
WIPO Patent Application WO/2016/076347
Kind Code:
A1
Abstract:
Provided is a novel material to be used in order to introduce an exogenous substance into a cell. Also provided is a method for introducing an exogenous substance into a cell of interest by utilizing this material. The present invention provides an exosome to be used in order to introduce an exogenous substance into a cell of interest, wherein the exosome includes therein one or more types of exogenous substance and a substance for inducing macropinocytosis in the cell of interest. The present invention also provides a composition including this exosome, and a method for introducing an exogenous substance into a cell using this exosome.
Inventors:
NAKASE IKUHIKO (JP)
YOSHIDA TETSUHIKO (JP)
BAILEYKOBAYASHI NAHOKO (JP)
YOSHIDA TETSUHIKO (JP)
BAILEYKOBAYASHI NAHOKO (JP)
Application Number:
PCT/JP2015/081709
Publication Date:
May 19, 2016
Filing Date:
November 11, 2015
Export Citation:
Assignee:
TOAGOSEI CO LTD (JP)
UNIV OSAKA PREFECT PUBLIC CORP (JP)
UNIV OSAKA PREFECT PUBLIC CORP (JP)
International Classes:
C12N5/07; A61K47/42; A61K47/46; A61P35/00; C12N15/09
Domestic Patent References:
| WO2011109677A2 | 2011-09-09 |
Other References:
OHNO,S. ET AL.: "Systemically injected exosomes targeted to EGFR deliver antitumor microRNA to breast cancer cells", MOL THER., vol. 1, 21 January 2013 (2013-01-21), pages 185 - 191
TANAKA,G. ET AL.: "CXCR4 stimulates macropinocytosis: implications for cellular uptake of arginine-rich cell - penetrating peptides and HIV", CHEM BIOL., vol. 19, no. 11, 21 November 2012 (2012-11-21), pages 1437 - 1446
NAKASE,I. ET AL.: "Active macropinocytosis induction by stimulation of epidermal growth factor receptor and oncogenic Ras expression potentiates cellular uptake efficacy of exosomes", SCI REP., vol. 5, 3 June 2015 (2015-06-03), pages 10300
IKUHIKO NAKASE ET AL.: "Macropinocytosis Yudo ni yoru Exosome no Saibonai Iko Sokushin", ABSTRACTS OF 37TH ANNUAL MEETING OF MEMBRANE SOCIETY OF JAPAN, 21 April 2015 (2015-04-21), pages 74
TANAKA,G. ET AL.: "CXCR4 stimulates macropinocytosis: implications for cellular uptake of arginine-rich cell - penetrating peptides and HIV", CHEM BIOL., vol. 19, no. 11, 21 November 2012 (2012-11-21), pages 1437 - 1446
NAKASE,I. ET AL.: "Active macropinocytosis induction by stimulation of epidermal growth factor receptor and oncogenic Ras expression potentiates cellular uptake efficacy of exosomes", SCI REP., vol. 5, 3 June 2015 (2015-06-03), pages 10300
IKUHIKO NAKASE ET AL.: "Macropinocytosis Yudo ni yoru Exosome no Saibonai Iko Sokushin", ABSTRACTS OF 37TH ANNUAL MEETING OF MEMBRANE SOCIETY OF JAPAN, 21 April 2015 (2015-04-21), pages 74
Attorney, Agent or Firm:
ABE MAKOTO (JP)
Makoto Abe (JP)
Makoto Abe (JP)
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