Title:
METHOD FOR INTRODUCING GENE TO EUGLENA, AND TRANSFORMANT THEREFROM
Document Type and Number:
WIPO Patent Application WO/2014/133159
Kind Code:
A1
Abstract:
Provided are a method for introducing a gene to Euglena, which can stably maintain a foreign gene, and a transformant therefrom. In this method for introducing a gene to Euglena, a DNA fragment containing an amino acid sequence for coding a protein is introduced into a Euglena cell. The method comprises: a step for producing a binary vector containing a DNA fragment; a step for obtaining a linear gene fragment comprising a T-DNA region including the DNA fragment in the binary vector; and a direct gene introduction step for directly introducing the linear gene fragment into the Euglena cell.
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Inventors:
SHIGEOKA SHIGERU (JP)
TAMOI MASAHIRO (JP)
SUZUKI KENGO (JP)
YOSHIDA ERIKO (JP)
TAMOI MASAHIRO (JP)
SUZUKI KENGO (JP)
YOSHIDA ERIKO (JP)
Application Number:
PCT/JP2014/055161
Publication Date:
September 04, 2014
Filing Date:
February 28, 2014
Export Citation:
Assignee:
EUGLENA CO LTD (JP)
UNIV KINKI (JP)
UNIV KINKI (JP)
International Classes:
C12N1/13; C12N15/09; C12N9/16
Domestic Patent References:
WO2011034946A1 | 2011-03-24 | |||
WO2012039159A1 | 2012-03-29 | |||
WO2008117537A1 | 2008-10-02 |
Foreign References:
JP2012502659A | 2012-02-02 | |||
JP2011512128A | 2011-04-21 | |||
JP2010029069A | 2010-02-12 | |||
JP2003501031A | 2003-01-14 | |||
JP2000253768A | 2000-09-19 | |||
JP2007525974A | 2007-09-13 | |||
JP2011512848A | 2011-04-28 |
Attorney, Agent or Firm:
AKIYAMA, Atsushi et al. (JP)
Akiyama 敦 (JP)
Akiyama 敦 (JP)
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