Title:
METHOD FOR INTRODUCING NUCLEIC ACID TO CELL AND NUCLEIC ACID COMPLEX
Document Type and Number:
WIPO Patent Application WO/2012/008361
Kind Code:
A1
Abstract:
Provided is a method for introducing a nucleic acid to a cell, and also provided is a nucleic acid complex used in the method. The method for introducing a nucleic acid to a cell comprises a step for forming a nucleic acid complex by bringing a double-stranded nucleic acid molecule into contact with a nucleic acid carrier, which has a peptide chain that forms a β-sheet structure in which a side chain of a basic amino acid having a positive charge is disposed on one surface side and a side chain of a hydrophobic amino acid is disposed on the opposite surface side, in the presence of a double-stranded nucleic acid molecule having a double helix structure and an amino acid sequence that alternates between a basic amino acid and a hydrophobic amino acid, and by joining the double-stranded nucleic acid molecule and the peptide chain by way of the electrostatic interaction between the side chains of the basic amino acid and phosphoric acid groups and also one or both hydrogen bonds.
Inventors:
FUJII MASAYUKI (JP)
Application Number:
PCT/JP2011/065612
Publication Date:
January 19, 2012
Filing Date:
July 07, 2011
Export Citation:
Assignee:
KITAKYUSHU FOUNDATION (JP)
FUJII MASAYUKI (JP)
FUJII MASAYUKI (JP)
International Classes:
C12N15/09
Foreign References:
| JP2005312397A | 2005-11-10 | |||
| JPH10146191A | 1998-06-02 | |||
| JP2003514564A | 2003-04-22 | |||
| JP2001288200A | 2001-10-16 | |||
| JPH10506001A | 1998-06-16 | |||
| JP2002316997A | 2002-10-31 | |||
| JPH11290073A | 1999-10-26 | |||
| JP2003503370A | 2003-01-28 | |||
| JP2004065238A | 2004-03-04 |
Other References:
DIALA, I. ET AL.: "Antisense inhibition of human telomerase by phosphorothioate oligonucleotide- peptide conjugates.", NUCLEIC ACIDS SYMP. SER., 2008, pages 679 - 680, XP055072890
MURAO, S. ET AL.: "Organic synthesis and antisense effects of oligonucleotide-peptide conjugates.", CURR. ORG. CHEM., vol. 13, no. 14, 2009, pages 1366 - 1377
DESHAYES S. ET AL.: "Delivery of proteins and nucleic acids using a non-covalent peptide- based strategy.", ADV. DRUG. DELIV. REV., vol. 60, no. 4-5, 2008, pages 537 - 547, XP022476844
EGUCHI A. ET AL.: "siRNA delivery using peptide transduction domains", TRENDS PHARMACOL. SCI., vol. 30, no. 7, 2009, pages 341 - 345, XP026250777
"Protocols that works well for gene transfection and expression analysis", September 2003, article "Kanarazu umaku iku idenshi donyu to hatugen kaiseki purotokoru"
PROC. NATL ACAD SCI USA, vol. 74, 1977, pages 1458 - 1462
See also references of EP 2594638A4
MURAO, S. ET AL.: "Organic synthesis and antisense effects of oligonucleotide-peptide conjugates.", CURR. ORG. CHEM., vol. 13, no. 14, 2009, pages 1366 - 1377
DESHAYES S. ET AL.: "Delivery of proteins and nucleic acids using a non-covalent peptide- based strategy.", ADV. DRUG. DELIV. REV., vol. 60, no. 4-5, 2008, pages 537 - 547, XP022476844
EGUCHI A. ET AL.: "siRNA delivery using peptide transduction domains", TRENDS PHARMACOL. SCI., vol. 30, no. 7, 2009, pages 341 - 345, XP026250777
"Protocols that works well for gene transfection and expression analysis", September 2003, article "Kanarazu umaku iku idenshi donyu to hatugen kaiseki purotokoru"
PROC. NATL ACAD SCI USA, vol. 74, 1977, pages 1458 - 1462
See also references of EP 2594638A4
Attorney, Agent or Firm:
KATO, Hisashi et al. (JP)
Hisashi Kato (JP)
Hisashi Kato (JP)
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