Title:
METHOD FOR ISOLATING ANIONIC ORGANIC SUBSTANCES FROM AQUEOUS SYSTEMS USING CATIONIC POLYMER NANOPARTICLES
Document Type and Number:
WIPO Patent Application WO1999010527
Kind Code:
A3
Abstract:
The invention relates to a method for isolating anionic organic substances from aqueous systems using polymer nanoparticles with cationic pH-sensitive surface groups. Extraction can be carried out directly from diluted solutions, biological media (blood plasma, serum, urine, etc.) and complex buffer systems (e.g. PCR preparations containing detergents) without prior derivatization of the samples or addition of binding buffers. After separation from the surrounding medium the conjugates of the corresponding substances and polymer nanoparticles obtained in this manner can be purified through additional washing steps and effectively desalinated. Owing to the pH-sensitivity of the basic surface groups, the bound substances can be released in a targeted manner after separation by modification of the pH of the medium. By using volatile bases contamination of the released samples with ionic residues can be avoided. By adding small quantities of SDS and acetonitrile during the separation stage, the sensitivity of the method can be raised further, especially when the substances to be isolated are present in low concentrations (<1 mu mol/L). The method is a method of extraction with universal application, suitable for both low-molecular anionic compounds as well as peptides, nucleic acids and nucleic acid derivatives. Recovery rates are up to 100 %.
Inventors:
BAYER ERNST (DE)
FRITZ HANS (DE)
MAIER MARTIN (US)
SCHEWITZ JENS (DE)
GERSTER MICHAEL (DE)
FRITZ HANS (DE)
MAIER MARTIN (US)
SCHEWITZ JENS (DE)
GERSTER MICHAEL (DE)
Application Number:
PCT/EP1998/005340
Publication Date:
May 20, 1999
Filing Date:
August 21, 1998
Export Citation:
Assignee:
SUEDDEUTSCHE KALKSTICKSTOFF (DE)
BAYER ERNST (DE)
FRITZ HANS (DE)
MAIER MARTIN (US)
SCHEWITZ JENS (DE)
GERSTER MICHAEL (DE)
BAYER ERNST (DE)
FRITZ HANS (DE)
MAIER MARTIN (US)
SCHEWITZ JENS (DE)
GERSTER MICHAEL (DE)
International Classes:
B01J41/04; C07B63/00; C07C37/82; C07C51/47; C07C55/14; C07C63/26; C07C201/16; C07C205/21; C07H1/06; C07H21/02; C07H21/04; C07K2/00; C12N15/10; C12Q1/68; C12Q1/6806; (IPC1-7): C07H1/06; C07C51/47; C07C55/14; C07C63/26; C07C37/82; C07C201/16
Foreign References:
| EP0281390A2 | 1988-09-07 | |||
| FR2691969A1 | 1993-12-10 |
Download PDF:
Previous Patent: METHODS FOR QUANTITATING HIGH-DENSITY LIPOPROTEIN CHOLESTEROL
Next Patent: ESTIMATION OF NUCLEIC ACID
Next Patent: ESTIMATION OF NUCLEIC ACID