Title:
METHOD OF ISOLATING CELLS
Document Type and Number:
WIPO Patent Application WO1997009600
Kind Code:
A3
Abstract:
The invention provides a method of isolating cells from a faecal stool, the method comprising the steps of a) cooling the stool to a temperature below its gel freezing point, and b) removing cells from the stool whilst maintaining the stool at a temperature below its gel freezing point such that the stool remains substantially intact. The invention further provides methods of purifying cells comprising use of immunomagnetic beads and/or boric acid. Cells isolated according to the invention may be used in diagnostic tests and assay procedures for monitoring a biological or biochemical property of tissue.
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Inventors:
O'NEILL IAN KENNETH (GB)
LOKTIONOV ALEXANDRE (GB)
LOKTIONOV ALEXANDRE (GB)
Application Number:
PCT/GB1996/002177
Publication Date:
June 26, 1997
Filing Date:
September 05, 1996
Export Citation:
Assignee:
MEDICAL RES COUNCIL (GB)
NEILL IAN KENNETH O (GB)
LOKTIONOV ALEXANDRE (GB)
NEILL IAN KENNETH O (GB)
LOKTIONOV ALEXANDRE (GB)
International Classes:
C12N5/07; C12N5/078; C12Q1/24; C12Q1/68; G01N33/553; C12Q1/6804; C12Q1/6806; C12Q1/6886; G01N1/12; G01N1/28; G01N1/42; G01N33/569; (IPC1-7): G01N1/30; G01N33/574; C12Q1/68; C12N1/00
Domestic Patent References:
| WO1993020235A1 | 1993-10-14 | |||
| WO1992017609A1 | 1992-10-15 |
Foreign References:
| US4971905A | 1990-11-20 |
Other References:
SIDRANSKY ET AL.: "Identification of ras oncogene mutations ....", SCIENCE, vol. 256, 3 April 1992 (1992-04-03), pages 102 - 104, XP000605488
ALBAUGH ET AL.: "Isolation of exfoliated colonic epithelial cells ....", INTERNATIONAL JOURNAL OF CANCER, vol. 52, 1992, NEW YORK NY, pages 347 - 350, XP000196502
HARDINGHAM ET AL.: "Immunobead-PCR: A technique for the detection of circulating tumor cells ....", CANCER RESEARCH, vol. 53, 1993, pages 3455 - 3458, XP000605489
ALBAUGH ET AL.: "Isolation of exfoliated colonic epithelial cells ....", INTERNATIONAL JOURNAL OF CANCER, vol. 52, 1992, NEW YORK NY, pages 347 - 350, XP000196502
HARDINGHAM ET AL.: "Immunobead-PCR: A technique for the detection of circulating tumor cells ....", CANCER RESEARCH, vol. 53, 1993, pages 3455 - 3458, XP000605489
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