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Title:
METHOD FOR ISOLATING EXOSOMES FROM BIOLOGICAL SOLUTIONS USING IRON OXIDE NANOPARTICLES
Document Type and Number:
WIPO Patent Application WO/2010/121335
Kind Code:
A1
Abstract:
A method is disclosed for isolating exosomes from blood platelets using superparamagnetic nanoparticles of iron oxide (Fe3O4), by means of a charge attraction mechanism based on the predetermined Zeta potential of the exosomes. The method involves the use of iron oxide nanoparticles that are previously synthesised with a predetermined positive charge, and that bond to the negatively charged exosomes contained in the biological sample. During incubation, the cationic magnetic nanoparticles are absorbed by the surface of the membrane of the exosomes owing to electrostatic interaction. Exposure of the material to a magnetic field makes it possible to separate the exosomes bonded to the nanoparticles. The success of this technique has been confirmed by characterisation of the exosomes by flow citometry. The method has been shown to be suitable for this purpose, since it allows exosomes to be isolated and purified, without undergoing alterations of their original morphological and structural characteristics.

Inventors:
CONTRERAS, Lionel Fernel Gamarra (Av. Prof. Mello Moraes, 1.235 - Ala 1, CEP: -030 São Paulo, 05508, BR)
GUILHEN, Daiane Doná (Rua Eugene Carriere, 30 - apt. 56 - Torre 01 - Jardim das Vertentes, CEP: -100 São Paulo, 05541, BR)
JANISZEWSKI, Mariano (Av. Morumbi, 8.264 - Brooklin, CEP: -002 São Paulo, 04703, BR)
MARTI, Luciana Cavalheiro (Rua Elizabeth Barbegian Baldinato, 18/92 - Vila Suzana, CEP: -070 São Paulo, 05630, BR)
PAVON, Lorena Favaro (Rua Maria Cândida, 660 - apt. 74b - Vila Guilherme, CEP: -011 São Paulo, 02071, BR)
Application Number:
BR2010/000032
Publication Date:
October 28, 2010
Filing Date:
January 28, 2010
Export Citation:
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Assignee:
SOCIEDADE BENEFICIENTE ISRAELITA BRASILEIRA HOSPITAL ALBERT EINSTEIN (Av. Albert Einstein, 627/701 - Morumbi, CEP: -000 Sao Paulo, 05652, BR)
CONTRERAS, Lionel Fernel Gamarra (Av. Prof. Mello Moraes, 1.235 - Ala 1, CEP: -030 São Paulo, 05508, BR)
GUILHEN, Daiane Doná (Rua Eugene Carriere, 30 - apt. 56 - Torre 01 - Jardim das Vertentes, CEP: -100 São Paulo, 05541, BR)
JANISZEWSKI, Mariano (Av. Morumbi, 8.264 - Brooklin, CEP: -002 São Paulo, 04703, BR)
MARTI, Luciana Cavalheiro (Rua Elizabeth Barbegian Baldinato, 18/92 - Vila Suzana, CEP: -070 São Paulo, 05630, BR)
PAVON, Lorena Favaro (Rua Maria Cândida, 660 - apt. 74b - Vila Guilherme, CEP: -011 São Paulo, 02071, BR)
International Classes:
B03C1/01; G01N27/447; G01N33/48
Domestic Patent References:
2001-05-25
2005-02-17
Foreign References:
DE102008040042A12009-12-31
Attorney, Agent or Firm:
SOMOS MARCAS E PATENTES LTDA. - LORIMARY G. MALHEIROS (Av. Paulista, 2.518 - cj. 132 - Cerqueira Cesar, CEP: -300 São Paulo, 01310, BR)
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