Title:
METHOD FOR ISOLATING EXTRACELLULAR VESICLE USING SALT FRACTIONAL PRECIPITATION
Document Type and Number:
WIPO Patent Application WO/2022/255840
Kind Code:
A1
Abstract:
The present invention relates to a method and kit for isolating an extracellular vesicle (EV) from a biological sample using fractional precipitation. According to the method and kit, rapid isolation is possible, and an EV can be effectively isolated from various types of biological samples regardless of the sample sources. Unlike a method for isolating an EV on the basis of an existing precipitation method, the present invention can easily remove a salt by washing, and, thus, has the benefit of being able to isolate a highly pure EV with a high yield rate and can be applied to both small-scale EV isolation in research and diagnostic fields and large-scale EV isolation for industrial applications.
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Inventors:
CHO YOON-KYOUNG (KR)
SUNKARA VIJAYA (KR)
SUNKARA VIJAYA (KR)
Application Number:
PCT/KR2022/007932
Publication Date:
December 08, 2022
Filing Date:
June 03, 2022
Export Citation:
Assignee:
INST BASIC SCIENCE (KR)
ULSAN NAT INST SCIENCE & TECH UNIST (KR)
ULSAN NAT INST SCIENCE & TECH UNIST (KR)
International Classes:
C12N5/00; B01D15/38; B01D21/00; B01D35/02; B01D61/14; C12N5/071; C12N5/078
Domestic Patent References:
| WO2017141947A1 | 2017-08-24 |
Foreign References:
| KR102215237B1 | 2021-02-15 | |||
| CN109355258A | 2019-02-19 | |||
| US9835626B2 | 2017-12-05 | |||
| KR20190012022A | 2019-02-08 | |||
| US9005888B2 | 2015-04-14 | |||
| US9671321B2 | 2017-06-06 | |||
| US20200179827A1 | 2020-06-11 | |||
| US9829483B2 | 2017-11-28 | |||
| US20190078078A1 | 2019-03-14 | |||
| US9835626B2 | 2017-12-05 |
Other References:
BALAJ ET AL., SCI REP, vol. 5, 2015, pages 10266
NAKAI ET AL., SCI REP, vol. 6, 2016, pages 33935
GHOSH ET AL., PLOS ONE, 2014
J IMMUNOL METHODS., vol. 407, 2014, pages 120 - 6
ANA GAMEZ-VALERO ET AL., FRONT. IMMUNOL., vol. 6, no. 6, 2015
WIGGINS, P. M., CELLULAR AND MOLECULAR BIOLOGY, vol. 47, 2001, pages 735 - 744
THERY ET AL., NAT. REV. IMMUNOL., vol. 2, 2002, pages 569 - 579
VALADI ET AL., NAT. CELL. BIOL., vol. 9, 2007, pages 654 - 659
J. IMMUNOL. MET., vol. 407, 2014, pages 120
NAKAI ET AL., SCI REP, vol. 6, 2016, pages 33935
GHOSH ET AL., PLOS ONE, 2014
J IMMUNOL METHODS., vol. 407, 2014, pages 120 - 6
ANA GAMEZ-VALERO ET AL., FRONT. IMMUNOL., vol. 6, no. 6, 2015
WIGGINS, P. M., CELLULAR AND MOLECULAR BIOLOGY, vol. 47, 2001, pages 735 - 744
THERY ET AL., NAT. REV. IMMUNOL., vol. 2, 2002, pages 569 - 579
VALADI ET AL., NAT. CELL. BIOL., vol. 9, 2007, pages 654 - 659
J. IMMUNOL. MET., vol. 407, 2014, pages 120
Attorney, Agent or Firm:
LEE, Cheo Young et al. (KR)
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