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Title:
METHOD FOR ISOLATING AND EXTRACTING ADIPOSE-DERIVED STEM CELLS FROM ADIPOSE TISSUE AND CULTURING SAME WITHOUT USING COLLAGENASE, AND KIT FOR ISOLATING AND EXTRACTING ADIPOSE-DERIVED STEM CELLS
Document Type and Number:
WIPO Patent Application WO/2019/168000
Kind Code:
A1
Abstract:
Adipose-derived stem cells are isolated and extracted from an adipose tissue without using collagenase. An adipose tissue is covered with a nonwoven fabric sheet having sufficient interfiber space and pressed by an appropriate force. Thus, the adipose tissue infiltrates among fibers of the nonwoven fabric and then comes into contact with fibers surrounding the same. By immersing the nonwoven fabric as such in a culture medium, a number of adipose-derived stem cells are allowed to crawl out from the adipose tissue and then adhered to the fiber surface.

Inventors:
SUNAMI Hiroshi (Faculty of Medicine 207, Aza-Uehara, Nishihara-cho, Nakagami-gu, Okinawa 15, 〒9030215, JP)
SHIMIZU Yusuke (Faculty of Medicine 207, Aza-Uehara, Nishihara-cho, Nakagami-gu, Okinawa 15, 〒9030215, JP)
FUTENMA Naoko (Faculty of Medicine 207, Aza-Uehara, Nishihara-cho, Nakagami-gu, Okinawa 15, 〒9030215, JP)
MAKITA Masashi (LTD. 3-17-43 Chigasaki Higashi, Tsuzuki-k, Yokohama-shi Kanagawa 33, 〒2240033, JP)
OSAKA Naoya (LTD. 3-17-43 Chigasaki Higashi, Tsuzuki-k, Yokohama-shi Kanagawa 33, 〒2240033, JP)
Application Number:
JP2019/007472
Publication Date:
September 06, 2019
Filing Date:
February 27, 2019
Export Citation:
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Assignee:
UNIVERSITY OF THE RYUKYUS (1 Aza-Senbaru, Nishihara-cho Nakagami-gu, Okinawa 13, 〒9030213, JP)
ORTHOREBIRTH CO., LTD. (3-17-43, Chigasaki Higashi Tsuzuki-ku, Yokohama-sh, Kanagawa 33, 〒2240033, JP)
International Classes:
C12N5/0775
Domestic Patent References:
WO2016159240A12016-10-06
WO2016121775A12016-08-04
WO2018235745A12018-12-27
Foreign References:
JP2004141052A2004-05-20
Other References:
6 November 2012 (2012-11-06), Retrieved from the Internet [retrieved on 20190326]
Attorney, Agent or Firm:
NINOMIYA Katsuyuki (IPEDGE International Patent Firm, CrossOffice Shinjuku Bldg. 402 1-12, Nishi-Shinjuku 7-chome, Shinjuku-k, Tokyo 23, 〒1600023, JP)
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