Title:
A METHOD FOR ISOLATING AND PURIFYING IMMUNO-MODULATING POLYPEPTIDE FROM COW PLACENTA
Document Type and Number:
WIPO Patent Application WO/2006/111060
Kind Code:
A1
Abstract:
The present invention provides a method for isolating and purifying immuno-modulating polypeptide from cow placenta, which is characterized by using the steps of anion-exchange chromatography, gel exclusion chromatography and reverse-phase high performance liquid chromatography to isolate and purify immuno-modulating polypeptide from cow placenta, identifying its activity of stimulating lymphocyte proliferation in vitro by MTT method, then determining its molecular weight by MALDI-TOF-MS, its isoelectric point by CIEF and its amino acid sequence with analyzer for protein sequencing. Since the obtained immuno-modulating polypeptide by the method according to the present invention has more than 90% purity, its bioactivity can reach medicinal standards.
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Inventors:
WANG YULIANG (CN)
SHENG QINGHAI (CN)
FANG XINPING (CN)
XIA WENSHUI (CN)
ZHAI HONGMEI (CN)
WEI PENG (CN)
SHENG QINGHAI (CN)
FANG XINPING (CN)
XIA WENSHUI (CN)
ZHAI HONGMEI (CN)
WEI PENG (CN)
Application Number:
PCT/CN2006/000219
Publication Date:
October 26, 2006
Filing Date:
February 14, 2006
Export Citation:
Assignee:
SHI JIA ZHUANG SANLU GROUP CO (CN)
WANG YULIANG (CN)
SHENG QINGHAI (CN)
FANG XINPING (CN)
XIA WENSHUI (CN)
ZHAI HONGMEI (CN)
WEI PENG (CN)
WANG YULIANG (CN)
SHENG QINGHAI (CN)
FANG XINPING (CN)
XIA WENSHUI (CN)
ZHAI HONGMEI (CN)
WEI PENG (CN)
International Classes:
C07K7/06; A61K35/50; A61K38/00; A61K38/08; A61P37/02; C07K1/14; C07K1/16; C07K1/18; C07K1/36; C07K2/00; C07K14/47
Foreign References:
RU2007420C1 | 1994-02-15 | |||
JPH06116286A | 1994-04-26 | |||
JPH0420289A | 1992-01-23 |
Other References:
SHENG Q. ET AL.: "Research on the beef placenta extraction and stimulation of fibroblast proliferation activity", JOURNAL OF WUXI UNIVERSITY OF LIGHT INDUSTRY, vol. 24, no. 1, January 2005 (2005-01-01), pages 39 - 40, XP008071892
LANG G. ET AL.: "Purification of hepatocyte growth factor from cow placenta", ACTA ACADEMIAE MEDICINAE QINGDAO UNIVERSITATIS, vol. 31, no. 4, 1995, pages 273 - 276
HUANG D. ET AL.: "The purification and identification of anticoagulation protein from human placenta", CHINESE PHARMACOLOGICAL BULLETIN, vol. 20, no. 4, April 2004 (2004-04-01), pages 468 - 471, XP008071810
ERIKSSON H. ET AL.: "Characterization of human placental alkaline phosphatase by activity and protein assays, capillary electrophoresis and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry", JOURNAL OF CHROMATOGRAPHY B, vol. 755, no. 1&2, 5 May 2001 (2001-05-05), pages 311 - 319, XP004234506
LANG G. ET AL.: "Purification of hepatocyte growth factor from cow placenta", ACTA ACADEMIAE MEDICINAE QINGDAO UNIVERSITATIS, vol. 31, no. 4, 1995, pages 273 - 276
HUANG D. ET AL.: "The purification and identification of anticoagulation protein from human placenta", CHINESE PHARMACOLOGICAL BULLETIN, vol. 20, no. 4, April 2004 (2004-04-01), pages 468 - 471, XP008071810
ERIKSSON H. ET AL.: "Characterization of human placental alkaline phosphatase by activity and protein assays, capillary electrophoresis and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry", JOURNAL OF CHROMATOGRAPHY B, vol. 755, no. 1&2, 5 May 2001 (2001-05-05), pages 311 - 319, XP004234506
Attorney, Agent or Firm:
CN-KNOWHOW INTELLECTUAL PROPERTY AGENT LIMITED (Fortune International Center No. 17 Daliushu Rd, Beijing 1, CN)
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