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Title:
A METHOD FOR ISOLATING AND PURIFYING IMMUNO-MODULATING POLYPEPTIDE FROM COW PLACENTA
Document Type and Number:
WIPO Patent Application WO/2006/111060
Kind Code:
A1
Abstract:
The present invention provides a method for isolating and purifying immuno-modulating polypeptide from cow placenta, which is characterized by using the steps of anion-exchange chromatography, gel exclusion chromatography and reverse-phase high performance liquid chromatography to isolate and purify immuno-modulating polypeptide from cow placenta, identifying its activity of stimulating lymphocyte proliferation in vitro by MTT method, then determining its molecular weight by MALDI-TOF-MS, its isoelectric point by CIEF and its amino acid sequence with analyzer for protein sequencing. Since the obtained immuno-modulating polypeptide by the method according to the present invention has more than 90% purity, its bioactivity can reach medicinal standards.

Inventors:
WANG YULIANG (CN)
SHENG QINGHAI (CN)
FANG XINPING (CN)
XIA WENSHUI (CN)
ZHAI HONGMEI (CN)
WEI PENG (CN)
Application Number:
PCT/CN2006/000219
Publication Date:
October 26, 2006
Filing Date:
February 14, 2006
Export Citation:
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Assignee:
SHI JIA ZHUANG SANLU GROUP CO (CN)
WANG YULIANG (CN)
SHENG QINGHAI (CN)
FANG XINPING (CN)
XIA WENSHUI (CN)
ZHAI HONGMEI (CN)
WEI PENG (CN)
International Classes:
C07K7/06; A61K35/50; A61K38/00; A61K38/08; A61P37/02; C07K1/14; C07K1/16; C07K1/18; C07K1/36; C07K2/00; C07K14/47
Foreign References:
RU2007420C11994-02-15
JPH06116286A1994-04-26
JPH0420289A1992-01-23
Other References:
SHENG Q. ET AL.: "Research on the beef placenta extraction and stimulation of fibroblast proliferation activity", JOURNAL OF WUXI UNIVERSITY OF LIGHT INDUSTRY, vol. 24, no. 1, January 2005 (2005-01-01), pages 39 - 40, XP008071892
LANG G. ET AL.: "Purification of hepatocyte growth factor from cow placenta", ACTA ACADEMIAE MEDICINAE QINGDAO UNIVERSITATIS, vol. 31, no. 4, 1995, pages 273 - 276
HUANG D. ET AL.: "The purification and identification of anticoagulation protein from human placenta", CHINESE PHARMACOLOGICAL BULLETIN, vol. 20, no. 4, April 2004 (2004-04-01), pages 468 - 471, XP008071810
ERIKSSON H. ET AL.: "Characterization of human placental alkaline phosphatase by activity and protein assays, capillary electrophoresis and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry", JOURNAL OF CHROMATOGRAPHY B, vol. 755, no. 1&2, 5 May 2001 (2001-05-05), pages 311 - 319, XP004234506
Attorney, Agent or Firm:
CN-KNOWHOW INTELLECTUAL PROPERTY AGENT LIMITED (Fortune International Center No. 17 Daliushu Rd, Beijing 1, CN)
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