Title:
A METHOD FOR ISOLATION AND CULTURE OF HUMAN EMBTYONIC STEM CELL
Document Type and Number:
WIPO Patent Application WO/2004/111206
Kind Code:
A1
Abstract:
The present invention relates to the isolation and culture of human embryonic stem cell. Which comprises the following steps: (1) planting the human blastula in embryo fibroblast feeder layer; (2) picking out the inner cell mass (ICMs) when its outward growing are finished, then removing the peripheral differentiation cell layer, dispersing the ICMs to pieces, and replanting it in new embryo fibroblast feeder layer. The said method omits the step of immunity operation, and makes the isolation process simply.
Inventors:
LU GUANGXIU (CN)
LIN GE (CN)
XIE CHANGQING (CN)
LIN GE (CN)
XIE CHANGQING (CN)
Application Number:
PCT/CN2003/000459
Publication Date:
December 23, 2004
Filing Date:
June 13, 2003
Export Citation:
Assignee:
CHANGSHA HUI LIN LIFE TECHNOLO (CN)
LU GUANGXIU (CN)
LIN GE (CN)
XIE CHANGQING (CN)
LU GUANGXIU (CN)
LIN GE (CN)
XIE CHANGQING (CN)
International Classes:
C12N5/0735; (IPC1-7): C12N5/06; A01K67/027; C12N5/00
Foreign References:
| CN1284993A | 2001-02-21 | |||
| CN1404526A | 2003-03-19 |
Attorney, Agent or Firm:
Changsha, Zonekey Patent Law Firm (Changsha, Hunan 1, CN)
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