Title:
METHOD AND ITS KIT FOR QUANTITATIVELY DETECTING SPECIFIC ANALYTE WITH SINGLE CAPTURING AGENT
Document Type and Number:
WIPO Patent Application WO/2006/128362
Kind Code:
A1
Abstract:
The invention provides a method and its kit for quantitatively detecting a specific analyte with a single capturing agent. The quantitative detection of a specific analyte with a single capturing agent comprises: firstly combining the tested analyte with a solid phase capturing agent, then labeling analyte which has been trapped by the capturing agent with a report molecule; secondly eluting the labeled analyte from the complex, recombining the tested analyte with a new solid phase capturing agent, and ascertaining the content of analyte by detecting the report molecule’s label signal. The kit of the invention comprises a capturing device, a detecting device, a report molecule for labeling and an analysis substance eluate. The advantages of the invention are the need of one single capturing agent, the capability of detecting for many analytes which can’t be tested at present, wide application, high sensibility and low noise. The invention can be applied to diagnosis, medical expertise, new medicine development, application of protein micro array and chip, and fundamental research.
Inventors:
SUN DONGXU (CN)
Application Number:
PCT/CN2006/001099
Publication Date:
December 07, 2006
Filing Date:
May 25, 2006
Export Citation:
Assignee:
SUN DONGXU (CN)
International Classes:
G01N33/543; C12Q1/68; G01N33/577
Domestic Patent References:
| WO2004016802A2 | 2004-02-26 |
Foreign References:
| CN1557965A | 2004-12-29 | |||
| CN1617936A | 2005-05-18 | |||
| US5573921A | 1996-11-12 | |||
| EP1347301A1 | 2003-09-24 | |||
| JP2004037394A | 2004-02-05 | |||
| JP2005035891A | 2005-02-10 |
Attorney, Agent or Firm:
NAN JING LAW OFFICE OF INTELLECTUAL PROPERTIES (Nanjing, Jiangsu 4, CN)
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