Title:
METHOD AND KIT FOR ANTIGEN DETECTION WITH REMOVED FALSE POSITIVE SIGNAL
Document Type and Number:
WIPO Patent Application WO/2023/191500
Kind Code:
A1
Abstract:
The present invention relates to a method and a kit for easily detecting biomarkers using a FRET effect between a capture strand attached to a capture antibody and a detection strand attached to a detection antibody, where false positive signals have been removed. The present invention provides a kit for antigen detection, with removed false positive signals, the kit comprising: a substrate; a capture antibody that can be attached to the substrate and equipped with a fluorophore-labeled capture strand; a detection antibody that is complementary to all or part of the base sequence of the capture strand and equipped with a fluorophore-labeled detection strand; and a buffer, wherein the buffer has an ion concentration that is less than the concentration at which the capture strand and detection strand can perform complementary binding.
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Inventors:
LEE JONG JIN (KR)
Application Number:
PCT/KR2023/004189
Publication Date:
October 05, 2023
Filing Date:
March 29, 2023
Export Citation:
Assignee:
JL MEDILABS INC (KR)
International Classes:
G01N21/64; G01N33/563; G01N33/58
Domestic Patent References:
| WO2017066784A1 | 2017-04-20 |
Foreign References:
| KR102195625B1 | 2020-12-28 | |||
| KR20190108023A | 2019-09-23 | |||
| US20200033251A1 | 2020-01-30 |
Other References:
EWA HEYDUK, BENJAMIN DUMMIT, YIE-HWA CHANG, TOMASZ HEYDUK: "Molecular Pincers: Antibody-Based Homogeneous Protein Sensors", ANALYTICAL CHEMISTRY, AMERICAN CHEMICAL SOCIETY, vol. 80, no. 13, 1 July 2008 (2008-07-01), pages 5152 - 5159, XP055061182, ISSN: 00032700, DOI: 10.1021/ac8004154
Attorney, Agent or Firm:
KWAK, Hyun Kyu (KR)
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