Title:
METHOD AND KIT COMPRISING 4,000 HUMAN PATHOGENIC TARGET GENES
Document Type and Number:
WIPO Patent Application WO/2017/193833
Kind Code:
A1
Abstract:
A method and kit comprising 4,000 human pathogenic target genes. The method comprises the following steps: fragmenting a DNA to obtain a DNA fragment, performing end repair, performing A-tailing, adding a linker, then performing pre-capture LM-PCR; mixing 4,000 human pathogenic target genes with a pre-capture LM-PCR product to obtain a mixed library sample, mixing and hybridizing a probe library specific to the 4,000 human pathogenic target genes with the mixed library sample, then performing magnetic bead capturing and washing of a DNA; and performing post-capture LM-PCR. The kit comprises a DNA end repairing reagent, A-tailing reagent, linker-adding reagent, LM-PCR reagent, and capture reagent. 4,000 human pathogenic genes are selected as targets and combined in the invention. A biotin probe capture technology is then used to perform one-time amplification and high-throughput sequencing at the same time, thereby allowing simultaneous screening for multiple mutant forms of the genes. The testing method has a high level of sensitivity, and can use 4,000 pathogenic genes as a primary clinical diagnostic tool. The method can reduce cost, reduce the likelihood of misdiagnosis, improve true positive rate of testing, and can be promoted in clinical settings.
Inventors:
ZHANG WEI VICTOR (CN)
Application Number:
PCT/CN2017/082496
Publication Date:
November 16, 2017
Filing Date:
April 28, 2017
Export Citation:
Assignee:
GUANGZHOU JIAJIAN MEDICAL TESTING CO LTD (CN)
International Classes:
C12N15/10
Foreign References:
| CN105925562A | 2016-09-07 | |||
| CN105907748A | 2016-08-31 | |||
| CN105861700A | 2016-08-17 | |||
| CN103198238A | 2013-07-10 | |||
| CN103806111A | 2014-05-21 | |||
| CN103103624A | 2013-05-15 | |||
| CN102796808A | 2012-11-28 |
Attorney, Agent or Firm:
ADVANCE CHINA IP LAW OFFICE (CN)
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