Title:
METHOD AND KIT FOR CULTURING HAIR FOLLICLE'S EPITHELIAL STEM CELLS
Document Type and Number:
WIPO Patent Application WO/2019/156032
Kind Code:
A1
Abstract:
Provided are a method and kit which are for culturing hair follicle's epithelial stem cells and which can grow a large amount of hair follicle's epithelial stem cells with hair regeneration capability thereof maintained. This method for culturing hair follicle's epithelial stem cells, comprises a mass formation step for disseminating hair follicle's epithelial stem cells in a cell culture vessel, and allowing the hair follicle's epithelial stem cells to form a mass, a mixing step for adding an extracellular matrix component to the mass of the hair follicle's epithelial stem cells, to produce a mixture of the extracellular matrix component and the mass of the hair follicle's epithelial stem cells, and a culture step for adding a culture medium to the mixture, and performing culture. This kit for culturing hair follicle's epithelial stem cells comprises a cell culture vessel made of an oxygen-permeable material, an extracellular matrix component, and a culture medium.
Inventors:
FUKUDA JUNJI (JP)
KAGEYAMA TATSUTO (JP)
HIRANO SUGI (JP)
KAGEYAMA TATSUTO (JP)
HIRANO SUGI (JP)
Application Number:
PCT/JP2019/003903
Publication Date:
August 15, 2019
Filing Date:
February 04, 2019
Export Citation:
Assignee:
NAT UNIV CORP YOKOHAMA NAT UNIV (JP)
KANAGAWA INSTITUTE OF INDUSTRIAL SCIENCE AND TECH (JP)
KANAGAWA INSTITUTE OF INDUSTRIAL SCIENCE AND TECH (JP)
International Classes:
C12N5/074; C12M3/00
Domestic Patent References:
| WO2017073625A1 | 2017-05-04 | |||
| WO2017070506A1 | 2017-04-27 | |||
| WO2017217393A1 | 2017-12-21 | |||
| WO2017073625A1 | 2017-05-04 |
Foreign References:
| JP2012249556A | 2012-12-20 | |||
| JP2009529886A | 2009-08-27 | |||
| JP2008125540A | 2008-06-05 | |||
| JPH10118103A | 1998-05-12 | |||
| JP2005218445A | 2005-08-18 | |||
| JP2012249556A | 2012-12-20 |
Other References:
CHACON-MARTINEZ, CARLOS ANDRES ET AL.: "Hair follicle stem cell cultures reveal self-organizing plasticity of stem cells and their progeny", THE EMBO JOURNAL, vol. 36, no. 2, 2017, pages 151 - 164, XP055629614, ISSN: 0261-4189, DOI: 10.15252/embj.201694902
CHACON-MARTINEZ C A ET AL.: "Hair follicle stem cell cultures reveal self-organizing plasticity of stem cells and their progeny", EMBO, vol. 36, no. 2, 2017, pages 151 - 164
See also references of EP 3750986A4
CHACON-MARTINEZ C A ET AL.: "Hair follicle stem cell cultures reveal self-organizing plasticity of stem cells and their progeny", EMBO, vol. 36, no. 2, 2017, pages 151 - 164
See also references of EP 3750986A4
Attorney, Agent or Firm:
HARUKA PATENT & TRADEMARK ATTORNEYS (JP)
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